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Hyperpolarization-activated cation currents in medium-size dorsal root ganglion cells are involved in overactive bladder syndrome in rats.
- Source :
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BMC urology [BMC Urol] 2020 Sep 02; Vol. 20 (1), pp. 140. Date of Electronic Publication: 2020 Sep 02. - Publication Year :
- 2020
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Abstract
- Background: To investigate the functions of the hyperpolarization-activated cation currents in medium-size dorsal root ganglion cells in a rat model of overactive bladder syndrome.<br />Methods: Rats with OAB were screened using a urodynamic testing device. The whole-cell patch clamp technique was used to investigate changes in excitability and hyperpolarization-activated cation current (I <subscript>h</subscript> ) of medium-size cells in the L6 dorsal root ganglia (DRG) of the OAB rats. Intrathecal injection of the specific I <subscript>h</subscript> inhibitor ZD7288 was used to investigate changes of voiding function and I <subscript>h</subscript> of medium-size cells in the L6 DRG.<br />Results: The urinary bladder weight of the OAB rats was significantly increased (p < 0.01); However, 7 days after intrathecally administration of ZD7288 (2 μM), the weight of rat bladder was significantly reduced (p < 0.01). The excitability of the medium-size cells in the L6 DRG of the OAB rats was significantly increased, and the number of action potentials elicited by a 500 pA stimulus was also markedly increased. Furthermore, ZD7288 significantly reduced the excitability of the medium-size DRG cells. The medium-size cells in the DRG of the OAB rats had a significantly increased I <subscript>h</subscript> current density, which was blocked by ZD7288.<br />Conclusions: The I <subscript>h</subscript> current density significantly increased in medium-size cells of the L6 DRG in the OAB model. A decrease of the I <subscript>h</subscript> current was able to significantly improve the voiding function of the OAB rats, in addition to lowering their urinary bladder weight. Our finding suggested that the observed increase of I <subscript>h</subscript> current in the medium-size DRG neurons might play an important role in the pathological processes of OAB.
Details
- Language :
- English
- ISSN :
- 1471-2490
- Volume :
- 20
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- BMC urology
- Publication Type :
- Academic Journal
- Accession number :
- 32878607
- Full Text :
- https://doi.org/10.1186/s12894-020-00698-z