Back to Search
Start Over
Platycodin D suppresses cisplatin-induced cytotoxicity by suppressing ROS-mediated oxidative damage, apoptosis, and inflammation in HEK-293 cells.
- Source :
-
Journal of biochemical and molecular toxicology [J Biochem Mol Toxicol] 2021 Jan; Vol. 35 (1), pp. e22624. Date of Electronic Publication: 2020 Sep 03. - Publication Year :
- 2021
-
Abstract
- Cisplatin, a proven effective chemotherapeutic agent, has been used clinically to treat malignant solid tumors, whereas its clinical use is limited by serious side effect including nephrotoxicity. Platycodin D (PD), the major and marked saponin isolated from Platycodon grandiflorum, possesses many pharmacological effects. In this study, we evaluated its protective effect against cisplatin-induced human embryonic kidney 293 (HEK-293) cells injury and elucidated the related mechanisms. Our results showed that PD (0.25, 0.5, and 1 μM) can dose-dependently alleviate oxidative stress by decreasing malondialdehyde and reactive oxygen species, while increasing the levels of glutathione, superoxide dismutase, and catalase. Moreover, the elevation of apoptosis including Bax, Bad, cleaved caspase-3,-9, and decreased protein levels of Bcl-2, Bcl-XL induced by cisplatin were reversed after PD treatment. Importantly, PD pretreatment can also regulate PI3K/Akt and ERK/JNK/p38 signaling pathways. Furthermore, PD was found to reduce NF-κB-mediated inflammatory relative proteins. Our finding indicated that PD exerted significant effects on cisplatin induced oxidative stress, apoptosis and inflammatory, which will provide evidence for the development of PD to attenuate cisplatin-induced nephrotoxicity.<br /> (© 2020 Wiley Periodicals LLC.)
- Subjects :
- Cisplatin pharmacology
HEK293 Cells
Humans
Inflammation chemically induced
Inflammation drug therapy
Inflammation metabolism
Inflammation pathology
Apoptosis drug effects
Cisplatin adverse effects
MAP Kinase Signaling System drug effects
Reactive Oxygen Species metabolism
Saponins pharmacology
Triterpenes pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1099-0461
- Volume :
- 35
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of biochemical and molecular toxicology
- Publication Type :
- Academic Journal
- Accession number :
- 32881195
- Full Text :
- https://doi.org/10.1002/jbt.22624