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Enhancement of the Peroxidase-Like Activity of Iodine-Capped Gold Nanoparticles for the Colorimetric Detection of Biothiols.
- Source :
-
Biosensors [Biosensors (Basel)] 2020 Sep 01; Vol. 10 (9). Date of Electronic Publication: 2020 Sep 01. - Publication Year :
- 2020
-
Abstract
- A colorimetric assay was developed for the detection of biothiols, based on the peroxidase-like activity of iodine-capped gold nanoparticles (AuNPs). These AuNPs show a synergetic effect in the form of peroxidase-mimicking activity at the interface of AuNPs, while free AuNPs and iodine alone have weak catalytic properties. Thus, iodine-capped AuNPs possess good intrinsic enzymatic activity and trigger the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB), leading to a change in color from colorless to yellow. When added to solution, biothiols, such as cysteine, strongly bind to the interface of AuNPs via gold-thiol bonds, inhibiting the catalytic activity of AuNPs, resulting in a decrease in oxidized TMB. Using this strategy, cysteine could be linearly determined, at a wide range of concentrations (0.5 to 20 μM), with a detection limit of 0.5 μM using UV-Vis spectroscopy. This method was applied for the detection of cysteine in diluted human urine.
Details
- Language :
- English
- ISSN :
- 2079-6374
- Volume :
- 10
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Biosensors
- Publication Type :
- Academic Journal
- Accession number :
- 32882936
- Full Text :
- https://doi.org/10.3390/bios10090113