Detection of Streptococcus pneumoniae antigen in pleural fluid: usefulness of an immunofluorescence-based lateral flow assay for the diagnosis of pneumococcal pneumonia.

Authors :: Romero Herrero D
Soler-Palacin P
Burgos Cibrian J
Falcó Ferrer V
Anton Pagarolas A
Martin-Gomez MT
Source :: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2020 Dec; Vol. 98 (4), pp. 115162. Date of Electronic Publication: 2020 Aug 05.
Publication Year :: 2020
Abstract: The performance of an immunofluorescence-based Streptococcus pneumoniae antigen detection test in pleural fluid (IF-PF) was evaluated. For proven and possible pneumococcal pneumonias global sensitivity and specificity were 92.6 (95 CI 76.6-97.9) and 80 (95 CI 62.7-90.5), respectively, with no significant differences between children and adults. Global diagnostic accuracy of IF-PF was 86% (74.2-93.7), and a substantial k index of concordance with culture/RT-PCR of 0.716 (0.535-0.896). IF-PF might be useful as a rapid complementary test for the etiologic diagnosis of pneumococcal pneumonia.<br /> (Copyright © 2020 Elsevier Inc. All rights reserved.)

Subjects :: Adolescent
Aged
Biosensing Techniques
Child
Child, Preschool
Female
Fluoroimmunoassay
Humans
Male
Middle Aged
Pleural Effusion diagnosis
Pleural Effusion microbiology
Retrospective Studies
Sensitivity and Specificity
Streptococcus pneumoniae isolation & purification
Antigens, Bacterial analysis
Bronchoalveolar Lavage Fluid microbiology
Pneumonia, Pneumococcal diagnosis
Streptococcus pneumoniae immunology

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