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Transcriptome Analysis of the Chicken Follicular Theca Cells with miR-135a-5p Suppressed.
- Source :
-
G3 (Bethesda, Md.) [G3 (Bethesda)] 2020 Nov 05; Vol. 10 (11), pp. 4071-4081. Date of Electronic Publication: 2020 Nov 05. - Publication Year :
- 2020
-
Abstract
- As a class of transcription regulators, numerous miRNAs have been verified to participate in regulating ovary follicular development in chickens ( Gallus gallus ). Previously we showed that gga-miR-135a-5p has significant differential expression between high and low-yield chicken ovaries, and the abundance of gga-miR-135a-5p is significantly higher in follicular theca cells than in granulosa cells. However, the exact role of gga-miR-135a-5p in chicken follicular theca cells is unclear. In this study, primary chicken follicular theca cells were isolated and then transfected with gga-miR-135a-5p inhibitor. Transcriptome sequencing was performed in chicken follicular theca cells with or without transfection. Differentially expressed genes (DEGs) were analyzed using bioinformatics. A dual-luciferase reporter assay was used to verify the target relationship between gga-miR-135a-5p and predicted targets within the DEGs. Compared with the normal chicken follicle theca cells, 953 up-regulated and 1060 down-regulated genes were detected in cells with gga-miR-135a-5p inhibited. The up-regulated genes were significantly enriched in Gene Ontology terms and pathways involved in cell proliferation and differentiation. In chicken follicular theca cells, Krüppel-like factor 4 (KLF4), ATPase phospholipid transporting 8A1 (ATP8A1), and Complexin-1 (CPLX1) were significantly up-regulated when the expression of gga-miR-135a-5p was inhibited. In addition, KLF4, ATP8A1, and CPLX1 confirmed as targets of gga-miR-135a-5p by using a dual-luciferase assay in vitro The results suggest that gga-mir-135a-5p may involve in proliferation and differentiation in chicken ovarian follicular theca cells by targeting KLF4, ATP8A1, and CPLX1.<br /> (Copyright © 2020 Zhou et al.)
Details
- Language :
- English
- ISSN :
- 2160-1836
- Volume :
- 10
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- G3 (Bethesda, Md.)
- Publication Type :
- Academic Journal
- Accession number :
- 32900904
- Full Text :
- https://doi.org/10.1534/g3.120.401701