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A bacteriophage infecting Mesorhizobium species has a prolate capsid and shows similarities to a family of Caulobacter crescentus phages.
- Source :
-
Canadian journal of microbiology [Can J Microbiol] 2021 Feb; Vol. 67 (2), pp. 147-160. Date of Electronic Publication: 2020 Sep 09. - Publication Year :
- 2021
-
Abstract
- Mesorhizobium phage vB_MloS_Cp1R7A-A1 was isolated from soil planted with chickpea in Saskatchewan. It is dissimilar in sequence and morphology to previously described rhizobiophages. It is a B3 morphotype virus with a distinct prolate capsid and belongs to the tailed phage family Siphoviridae . Its genome has a GC content of 60.3% and 238 predicted genes. Putative functions were predicted for 57 genes, which include 27 tRNA genes with anticodons corresponding to 18 amino acids. This represents the highest number of tRNA genes reported yet in a rhizobiophage. The gene arrangement shows a partially modular organization. Most of the structural genes are found in one module, whereas tRNA genes are in another. Genes for replication, recombination, and nucleotide metabolism form the third module. The arrangement of the replication module resembles the replication module of Enterobacteria phage T5, raising the possibility that it uses a recombination-based replication mechanism, but there is also a suggestion that a T7-like replication mechanism could be used. Phage termini appear to be long direct repeats of just over 12 kb in length. Phylogenetic analysis revealed that Cp1R7A-A1 is more closely related to PhiCbK-like Caulobacter phages and other B3 morphotype phages than to other rhizobiophages sequenced thus far.
- Subjects :
- Bacteriophages classification
Bacteriophages genetics
Bacteriophages ultrastructure
Caulobacter crescentus virology
Genes, Viral genetics
Genome, Viral genetics
Phylogeny
Siphoviridae classification
Siphoviridae genetics
Siphoviridae ultrastructure
Species Specificity
Bacteriophages isolation & purification
Capsid ultrastructure
Mesorhizobium virology
Phosmet
Siphoviridae isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1480-3275
- Volume :
- 67
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Canadian journal of microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 32905709
- Full Text :
- https://doi.org/10.1139/cjm-2020-0281