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Effects of Acanthamoeba castellanii on the dissolved oxygen and the microbial community under the experimental aquatic model.
- Source :
-
Experimental parasitology [Exp Parasitol] 2020 Nov; Vol. 218, pp. 107985. Date of Electronic Publication: 2020 Sep 09. - Publication Year :
- 2020
-
Abstract
- Acanthamoeba castellanii is a protist that has a high predation efficiency for bacteria in a number of monoxenic culture experiments. However, the role of A. castellanii in the microbial community is still unknown because of the lack of studies on multiple-species interactions. The aim of this study was to investigate the change of bacterial composition after A. castellanii emerges in a water environment. We added A. castellanii to an environmental water sample and incubated it for two days. Then, we performed 16S ribosomal RNA sequencing techniques to analyze the changes in bacterial composition. In this study, A. castellanii slightly increased the relative abundance of a few opportunistic pathogens, such as Legionella, Roseomonas, and Haemophilus. This result may be related to the training ground hypothesis. On the other hand, the growth of some bacteria was inhibited, such as Cyanobacteria and Firmicutes. Although A. castellanii did not drastically change the whole bacterial community, we surprisingly found the dissolved oxygen concentration was increased after incubation with A. castellanii. We applied environmental water at the laboratory scale to investigate the interactions among A. castellanii, complex microbial communities and the environment. We identified the bacteria that are sensitive to A. castellanii and further found the novel relationship between dissolved oxygen and microbial interaction. Our results helped to clarify the role of A. castellanii in microbial communities.<br /> (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Subjects :
- Acanthamoeba castellanii genetics
Bdellovibrio genetics
Bdellovibrio physiology
DNA isolation & purification
Legionella genetics
Legionella pathogenicity
Legionella physiology
Ponds microbiology
Ponds parasitology
RNA, Ribosomal, 16S chemistry
Real-Time Polymerase Chain Reaction
Reproducibility of Results
Virulence
Acanthamoeba castellanii physiology
Bdellovibrio isolation & purification
Legionella isolation & purification
Microbiota physiology
Oxygen metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2449
- Volume :
- 218
- Database :
- MEDLINE
- Journal :
- Experimental parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 32918877
- Full Text :
- https://doi.org/10.1016/j.exppara.2020.107985