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Effect of uremic serum on Th17/Treg cell balance and endoplasmic reticulum stress in rats.
- Source :
-
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2020 Nov; Vol. 131, pp. 110705. Date of Electronic Publication: 2020 Sep 09. - Publication Year :
- 2020
-
Abstract
- Background/aims: The imbalance of T helper 17 (Th17) and regulatory T (Treg) cells exists in the occurrence and development of various diseases. Endoplasmic reticulum stress (ERS) is an important self-protective cellular response to harmful stimuli, such as uremic environment. The objective of this study was to investigate the Th17/Treg cell balance and ERS in a uremic environment and analyze the relationship between them.<br />Methods: (1) The rat spleen lymphocytes were extracted and treated with thapsigargin (inducer of ERS) and sodium citrate. The proportion of Th17 and Treg cells were then detected. (2) The uremic serum-cultured lymphocytes were used and divided into three groups: non-uremic serum group, uremic serum group, and uremic serum + sodium citrate group. Afterward, the proportion of Th17/Treg cells and the expression of ERS-related proteins (GRP78 and CHOP) were detected.<br />Results: Thapsigargin had no significant effect on the proportion of Th17 cells within a limited concentration range, but it could reduce the proportion of Treg cells, sodium citrate had a negative influence on the deviation of Th17/Treg cells treated with thapsigargin. Uremic serum treatment reduced the proportion of Treg cells, resulting in an increase of the Th17/Treg ratio. However, sodium citrate had no influence on the deviation of Th17/Treg cells treated by uremic serum. Sodium citrate reduced the elevation of ERS-related proteins induced by uremic serum.<br />Conclusions: Uremic serum can lead to the imbalance of Th17/Treg cells as well as ERS, suggesting that ERS is one of the mechanisms of the imbalance of Th17/Treg cells induced by uremic serum. Sodium citrate can inhibit ERS induced by uremic serum.<br /> (Copyright © 2020 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
- Subjects :
- Adult
Aged
Animals
Cells, Cultured
Endoplasmic Reticulum Chaperone BiP
Endoplasmic Reticulum Stress drug effects
Heat-Shock Proteins analysis
Humans
Lactones pharmacology
Middle Aged
Rats
Rats, Sprague-Dawley
Sesquiterpenes pharmacology
Sodium Citrate pharmacology
T-Lymphocytes, Regulatory drug effects
Th17 Cells drug effects
Transcription Factor CHOP analysis
Endoplasmic Reticulum Stress physiology
T-Lymphocytes, Regulatory immunology
Th17 Cells immunology
Uremia blood
Subjects
Details
- Language :
- English
- ISSN :
- 1950-6007
- Volume :
- 131
- Database :
- MEDLINE
- Journal :
- Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
- Publication Type :
- Academic Journal
- Accession number :
- 32919190
- Full Text :
- https://doi.org/10.1016/j.biopha.2020.110705