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Trim24 and Trim33 Play a Role in Epigenetic Silencing of Retroviruses in Embryonic Stem Cells.
- Source :
-
Viruses [Viruses] 2020 Sep 11; Vol. 12 (9). Date of Electronic Publication: 2020 Sep 11. - Publication Year :
- 2020
-
Abstract
- Embryonic stem cells (ESC) have the ability to epigenetically silence endogenous and exogenous retroviral sequences. Trim28 plays an important role in establishing this silencing, but less is known about the role other Trim proteins play. The Tif1 family is a sub-group of the Trim family, which possess histone binding ability in addition to the distinctive RING domain. Here, we have examined the interaction between three Tif1 family members, namely Trim24, Trim28 and Trim33, and their function in retroviral silencing. We identify a complex formed in ESC, comprised of these three proteins. We further show that when Trim33 is depleted, the complex collapses and silencing efficiency of both endogenous and exogenous sequences is reduced. Similar transcriptional activation takes place when Trim24 is depleted. Analysis of the H3K9me3 chromatin modification showed a decrease in this repressive mark, following both Trim24 and Trim33 depletion. As Trim28 is an identified binding partner of the H3K9 methyltransferase ESET, this further supports the involvement of Trim28 in the complex. The results presented here suggest that a complex of Tif1 family members, each of which possesses different specificity and efficiency, contributes to the silencing of retroviral sequences in ESC.
- Subjects :
- Animals
Apoptosis Regulatory Proteins
Carrier Proteins genetics
Embryonic Stem Cells cytology
Embryonic Stem Cells virology
Endogenous Retroviruses
HEK293 Cells
Histones metabolism
Humans
Leukemia Virus, Murine
Mice
NIH 3T3 Cells
Nuclear Proteins
Protein Binding
Transcription Factors genetics
Tripartite Motif-Containing Protein 28 metabolism
Carrier Proteins metabolism
Embryonic Stem Cells metabolism
Epigenesis, Genetic physiology
Gene Silencing
Retroviridae genetics
Retroviridae physiology
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1999-4915
- Volume :
- 12
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Viruses
- Publication Type :
- Academic Journal
- Accession number :
- 32932986
- Full Text :
- https://doi.org/10.3390/v12091015