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REST-repressed lncRNA NPPA-AS1 regulates cervical cancer progression by modulating miR-302e/DKK1/Wnt/β-catenin signaling pathway.
- Source :
-
Journal of cellular biochemistry [J Cell Biochem] 2021 Jan; Vol. 122 (1), pp. 16-28. Date of Electronic Publication: 2020 Sep 23. - Publication Year :
- 2021
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Abstract
- Long noncoding RNAs (lncRNAs), a group of transcripts, have been revealed to be critical participants in regulating multiple biological processes of malignant tumors. The knowledge of NPPA-AS1 (a new lncRNA) in cancer research is hardly known. Thus, it is of urgent need to study the underlying role of NPPA antisense RNA 1 (NPPA-AS1) in cervical cancer (CC). In this study, NPPA-AS1 was discovered to be lowly expressed and upregulation of it impaired cell proliferation and migration in CC. Besides, downregulation of it led to opposite results. Molecular mechanism assays uncovered that increased expression of NPPA-AS1 could inactivate Wnt/β-catenin signaling pathway in CC. In addition, NPPA-AS1 was found to negatively interact with miR-302e whereas positively correlate with dickkopf-1 (DKK1, an inhibitor of Wnt pathway) in CC. Besides, loss of function assay illuminated that miR-302e inhibition restrained cell proliferation and migration in CC. Subsequent rescue assays confirmed that NPPA-AS1 acted as a competing endogenous RNA in CC by sponging miR-302e to upregulate DKK1 expression. Finally, the RE-1 silencing transcription factor (REST) was testified to function as a transcription suppressor of NPPA-AS1 in CC. In brief, REST-repressed NPPA-AS1 regulates CC progression by modulating miR-302e/DKK1/Wnt/β-catenin signaling pathway.<br /> (© 2020 Wiley Periodicals LLC.)
- Subjects :
- Animals
Apoptosis
Atrial Natriuretic Factor antagonists & inhibitors
Atrial Natriuretic Factor genetics
Biomarkers, Tumor genetics
Biomarkers, Tumor metabolism
Cell Proliferation
Female
Humans
Intercellular Signaling Peptides and Proteins genetics
Male
Mice
Mice, Nude
MicroRNAs genetics
Middle Aged
Prognosis
RNA, Antisense genetics
Repressor Proteins genetics
Survival Rate
Tumor Cells, Cultured
Uterine Cervical Neoplasms genetics
Uterine Cervical Neoplasms metabolism
Wnt1 Protein genetics
Xenograft Model Antitumor Assays
beta Catenin genetics
Gene Expression Regulation, Neoplastic
Intercellular Signaling Peptides and Proteins metabolism
RNA, Long Noncoding genetics
Repressor Proteins metabolism
Uterine Cervical Neoplasms pathology
Wnt1 Protein metabolism
beta Catenin metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1097-4644
- Volume :
- 122
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of cellular biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 32965043
- Full Text :
- https://doi.org/10.1002/jcb.29701