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Design, synthesis and characterization of a fluorescently labeled functional analog of full-length human ghrelin.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2020 Dec 10; Vol. 533 (3), pp. 559-564. Date of Electronic Publication: 2020 Sep 23. - Publication Year :
- 2020
-
Abstract
- Human ghrelin receptor (GHSR) is a recognized prospective target in the diagnosis and therapy of multiple cancer types. To gain a better understanding of this receptor signaling system, we have synthesized a novel full-length ghrelin analog that is fluorescently labeled at the side-chain of a C-terminal cysteine extension. This analog exhibited nanomolar affinity and potency for the ghrelin receptor. It shows comparable efficacy with that of endogenous ghrelin. The fluorescently-labeled ghrelin analog is a valuable tool for in vitro imaging of cell lines that express ghrelin receptor.<br />Competing Interests: Declaration of competing interest The authors declare no conflict of interest.<br /> (Copyright © 2020 Elsevier Inc. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 533
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 32980116
- Full Text :
- https://doi.org/10.1016/j.bbrc.2020.09.028