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MiR-155-5p promotes renal interstitial fibrosis in obstructive nephropathy via inhibiting SIRT1 signaling pathway.
- Source :
-
Journal of receptor and signal transduction research [J Recept Signal Transduct Res] 2021 Oct; Vol. 41 (5), pp. 466-475. Date of Electronic Publication: 2020 Sep 26. - Publication Year :
- 2021
-
Abstract
- Protection against renal fibrosis is important for the management of obstructive nephropathy. We researched the roles and possible mechanism of miR-155-5p in renal interstitial fibrosis, which may provide a potential endogenous target for renal interstitial fibrosis in obstructive nephropathy. Herein, NRK-49F cells were transfected with miR-155-5p mimic, miR-155-5p inhibitor, SIRT1 plasmid and/or SIRT1 siRNA. The unilateral ureteral obstruction (UUO) model was built with male C57 black mice and administrated with SRT1720 by tail vein injection. Levels of miR-155-5p, SIRT1 and relative proteins (TGF-β1, α-SMA, Collage I and fibronectin) in NRK-49F cells or mice kidney tissues were measured with quantitative reverse transcription polymerase chain reaction or Western blot. The target gene of miR-155-5p was analyzed through TargetScan and dual-luciferase reporter assay. Mice kidney tissue was stained with Masson trichrome. It was found that miR-155-5p overexpression promoted the expressions of fibroblast related proteins expression and inhibited the SIRT1 expression in NRK-49F cells, while miR-155-5p silencing had an opposite effect. SIRT1 can bind with miR-155-5p. MiR-155-5p inhibited the level of SIRT1. Fibroblast related proteins were up-regulated by miR-155-5p and down-regulated by SIRT1 in NRK-49F cells, while the up-regulatory effect of miR-155-5p was reversed by SIRT1. MiR-155-5p expression was up-regulated and SIRT1 expression was down-regulated in the kidney tissue of UUO mice. SRT1720 attenuated the fiber deposition, up-regulated SIRT1 level and down-regulated the levels of fibroblast related proteins in UUO model mice. To conclude, miR-155-5p promotes renal interstitial fibrosis in obstructive nephropathy via inhibiting SIRT1 signaling pathway.
- Subjects :
- Animals
Apoptosis
Cell Proliferation
Cells, Cultured
Fibrosis etiology
Fibrosis metabolism
Kidney Diseases etiology
Kidney Diseases metabolism
Male
Mice
Mice, Inbred C57BL
Sirtuin 1 genetics
Sirtuin 1 metabolism
Fibrosis pathology
Gene Expression Regulation
Kidney Diseases pathology
MicroRNAs genetics
Sirtuin 1 antagonists & inhibitors
Ureteral Obstruction physiopathology
Subjects
Details
- Language :
- English
- ISSN :
- 1532-4281
- Volume :
- 41
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of receptor and signal transduction research
- Publication Type :
- Academic Journal
- Accession number :
- 32985331
- Full Text :
- https://doi.org/10.1080/10799893.2020.1825491