Improved Anti-Prion Nucleic Acid Aptamers by Incorporation of Chemical Modifications.

Authors :: Amato J
Mashima T
Kamatari YO
Kuwata K
Novellino E
Randazzo A
Giancola C
Katahira M
Pagano B
Source :: Nucleic acid therapeutics [Nucleic Acid Ther] 2020 Dec; Vol. 30 (6), pp. 414-421. Date of Electronic Publication: 2020 Sep 29.
Publication Year :: 2020
Abstract: Nucleic acid aptamers are innovative and promising candidates to block the hallmark event in the prion diseases, that is the conversion of prion protein (PrP) into an abnormal form; however, they need chemical modifications for effective therapeutic activity. This communication reports on the development and biophysical characterization of a small library of chemically modified G-quadruplex-forming aptamers targeting the cellular PrP and the evaluation of their anti-prion activity. The results show the possibility of enhancing anti-prion aptamer properties through straightforward modifications.

Subjects :: Animals
Binding Sites drug effects
Humans
Nucleic Acids chemistry
Nucleic Acids pharmacology
Prion Diseases genetics
Prion Diseases pathology
Prions genetics
Protein Binding drug effects
Aptamers, Nucleotide pharmacology
Prion Diseases drug therapy
Prions antagonists & inhibitors
SELEX Aptamer Technique

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