Back to Search
Start Over
Lower DNA methylation levels in CpG island shores of CR1, CLU, and PICALM in the blood of Japanese Alzheimer's disease patients.
- Source :
-
PloS one [PLoS One] 2020 Sep 29; Vol. 15 (9), pp. e0239196. Date of Electronic Publication: 2020 Sep 29 (Print Publication: 2020). - Publication Year :
- 2020
-
Abstract
- The aim of the present study was to (1) investigate the relationship between late-onset Alzheimer's disease (AD) and DNA methylation levels in six of the top seven AD-associated genes identified through a meta-analysis of recent genome wide association studies, APOE, BIN1, PICALM, CR1, CLU, and ABCA7, in blood, and (2) examine its applicability to the diagnosis of AD. We examined methylation differences at CpG island shores in the six genes using Sanger sequencing, and one of two groups of 48 AD patients and 48 elderly controls was used for a test or replication analysis. We found that methylation levels in three out of the six genes, CR1, CLU, and PICALM, were significantly lower in AD subjects. The combination of CLU methylation levels and the APOE genotype classified AD patients with AUC = 0.84 and 0.80 in the test and replication analyses, respectively. Our study implicates methylation differences at the CpG island shores of AD-associated genes in the onset of AD and suggests their diagnostic value.<br />Competing Interests: The authors have declared that no competing interests exist. K.S. is affiliated with Axcelead Drug Discovery Partners, Inc., Fujisawa, Kanagawa, Japan. This does not alter our adherence to PLOS ONE policies on sharing data and materials.
- Subjects :
- Aged
Alzheimer Disease diagnosis
Biomarkers blood
Female
Genetic Association Studies
Genetic Predisposition to Disease
Humans
Japan
Male
Middle Aged
Alzheimer Disease genetics
Clusterin blood
Clusterin genetics
CpG Islands
DNA Methylation
Monomeric Clathrin Assembly Proteins blood
Monomeric Clathrin Assembly Proteins genetics
Receptors, Complement 3b blood
Receptors, Complement 3b genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 15
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 32991610
- Full Text :
- https://doi.org/10.1371/journal.pone.0239196