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miR-200 family expression during normal and abnormal lung development due to congenital diaphragmatic hernia at the later embryonic stage in the nitrofen rat model.
- Source :
-
Pediatric surgery international [Pediatr Surg Int] 2020 Dec; Vol. 36 (12), pp. 1429-1436. Date of Electronic Publication: 2020 Oct 13. - Publication Year :
- 2020
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Abstract
- Introduction: Congenital diaphragmatic hernia (CDH) is a life-threatening disease associated with pulmonary hypoplasia. CDH occurs approximately 1 in every 2000-3000 live births, and the pathophysiology is unknown. MicroRNAs are short, non-coding RNAs that control gene expression through post-transcriptional regulation. Based on our previous work, we hypothesized that the miR-200 family is differentially expressed in normal and abnormal lung development. We aimed to examine the expression of the miR-200 family during normal and hypoplastic lung development due to CDH.<br />Methods: We performed reverse transcriptase polymerase chain reaction (RT-qPCR) and fluorescent in situ hybridization (FISH) to study the expression levels and distribution of the miR-200 family members on embryonic day 21 (E21) rat control and nitrofen-induced hypoplastic CDH lungs.<br />Results: RT-qPCR showed up-regulation of miR-200a in hypoplastic CDH lungs. FISH showed contrasting expression patterns for miR- 200a, miR-200c, and miR-429 between control and hypoplastic CDH lungs, while we could not detect miR-141 in control and hypoplastic CDH lungs.<br />Conclusion: We demonstrate a specific expression pattern of miR-200 family members in hypoplastic CDH lungs different from control lungs. This study suggests that disruption of miR-200 family expression plays a role in the pathogenesis of pulmonary hypoplasia associated with CDH.
- Subjects :
- Animals
Disease Models, Animal
Female
Hernias, Diaphragmatic, Congenital pathology
In Situ Hybridization, Fluorescence
Lung pathology
Phenyl Ethers
Rats
Rats, Sprague-Dawley
Real-Time Polymerase Chain Reaction
Gene Expression genetics
Hernias, Diaphragmatic, Congenital embryology
Hernias, Diaphragmatic, Congenital genetics
Lung embryology
MicroRNAs genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1437-9813
- Volume :
- 36
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Pediatric surgery international
- Publication Type :
- Academic Journal
- Accession number :
- 33048239
- Full Text :
- https://doi.org/10.1007/s00383-020-04757-2