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Host gene expression modulated by Zika virus infection of human-293 cells.
- Source :
-
Virology [Virology] 2021 Jan 02; Vol. 552, pp. 32-42. Date of Electronic Publication: 2020 Oct 06. - Publication Year :
- 2021
-
Abstract
- The HEK-293 cell line was created in 1977 by transformation of primary human embryonic kidney cells with sheared adenovirus type 5 DNA. A previous study determined that the HEK-293 cells have neuronal markers rather than kidney markers. In this study, we tested the hypothesis whether Zika virus (ZIKV), a neurotropic virus, is able to infect and replicate in the HEK-293 cells. We show that the HEK-293 cells infected with ZIKV support viral replication as shown by indirect immunofluorescence (IFA) and quantitative reverse transcriptase-PCR (qRT-PCR). We performed RNA-seq analysis on the ZIKV-infected and the control uninfected HEK-293 cells and find 659 genes that are differentially transcribed in ZIKV-infected HEK-293 cells as compared to uninfected cells. The results show that the top 10 differentially transcribed and upregulated genes are involved in antiviral and inflammatory responses. Seven upregulated genes, IFNL1, DDX58, CXCL10, ISG15, KCNJ15, IFNIH1, and IFIT2, were validated by qRT-PCR. Altogether, our findings show that ZIKV infection alters host gene expression by affecting their antiviral and inflammatory responses.<br /> (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Subjects :
- Apoptosis Regulatory Proteins metabolism
Chemokine CXCL10 metabolism
Cytokines metabolism
DEAD Box Protein 58 metabolism
Fluorescent Antibody Technique, Indirect methods
HEK293 Cells
Host Microbial Interactions
Humans
Interferon-Induced Helicase, IFIH1 metabolism
Interferons metabolism
Interleukins metabolism
Potassium Channels, Inwardly Rectifying metabolism
RNA-Binding Proteins metabolism
RNA-Seq
Receptors, Immunologic metabolism
Ubiquitins metabolism
Zika Virus immunology
Zika Virus Infection immunology
Gene Expression Regulation
Inflammation virology
Zika Virus metabolism
Zika Virus Infection metabolism
Zika Virus Infection virology
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0341
- Volume :
- 552
- Database :
- MEDLINE
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 33059318
- Full Text :
- https://doi.org/10.1016/j.virol.2020.09.007