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MicroRNAs of Human Herpesvirus 6A and 6B in Serum and Cerebrospinal Fluid of Multiple Sclerosis Patients.
- Source :
-
Frontiers in immunology [Front Immunol] 2020 Sep 18; Vol. 11, pp. 2142. Date of Electronic Publication: 2020 Sep 18 (Print Publication: 2020). - Publication Year :
- 2020
-
Abstract
- Human herpesvirus-6A (HHV-6A) and -6B (HHV-6B) might be involved in the etiopathogenesis of multiple sclerosis (MS), especially the HHV-6A. We aim at assessing, for the first time in the scientific literature, the HHV-6A/B microRNAs in MS patients. We analyzed the miRNAs of HHV-6A: miR-U86, and -6B: hhv6b-miR-Ro6-1, -2, -3-3p, -3-5p, and -4 in paired samples of serum and CSF of 42 untreated MS patients and 23 patients with other neurological diseases (OND), using Taqman MicroRNA Assays. Intrathecal HHV-6A/B antibody production and anti-HHV-6A/B IgG/IgM levels in serum were measured. MS clinical data were available. We detected the following miRNAs: hhv6b-miR-Ro6-2 (serum: MS:97.7%, OND:95.7%; CSF: MS:81%, OND:86.4%), 3-3p (serum: MS:4.8%, OND:0%; CSF: MS:2.4%, OND:4.5%), -3-5p (serum: MS:95.2%, OND:91.3%; CSF: MS:50%, OND:54.5%), and miR-U86 (serum: MS:54.8%, OND:47.8%; CSF: MS:11.9%, OND:9.1%). In the serum of the whole population (MS and OND patients) we found a significant correlation between the levels of hhv6b-miR-Ro6-2 and -3-5p (Spearman r = 0.839, pcorr = 3E-13), -2 and miR-U86 (Spearman r = 0.578, pcorr = 0.001) and -3-5p and miR-U86 (Spearman r = 0.698, pcorr = 1.34E-5); also in the CSF, between hhv6b-miR-Ro6-2 and -3-5p (Spearman r = 0.626, pcorr = 8.52E-4). These correlations remained statistically significant when both populations were considered separately. The anti-HHV-6A/B IgG levels in CSF and the intrathecal antibody production in positive MS patients for hhv6b-miR-Ro6-3-5p were statistically significant higher than in the negative ones (pcorr = 0.006 and pcorr = 0.036). The prevalence of miR-U86 (30.8%) in the CSF of individuals without gadolinium-enhancing lesions was higher ( p = 0.035) than in the ones with these lesions (0%); however, the difference did not withstand Bonferroni correction (pcorr = 0.105). We propose a role of HHV-6A/B miRNAs in the maintenance of the viral latency state. Further investigations are warranted to validate these results and clarify the function of these viral miRNAs.<br /> (Copyright © 2020 Domínguez-Mozo, Nieto-Guerrero, Pérez-Pérez, García-Martínez, Arroyo and Álvarez-Lafuente.)
- Subjects :
- Adult
Antibodies, Viral blood
Antibodies, Viral cerebrospinal fluid
Case-Control Studies
Female
Herpesvirus 6, Human genetics
Herpesvirus 6, Human immunology
Humans
Immunoglobulin G blood
Immunoglobulin G cerebrospinal fluid
Immunoglobulin M blood
Immunoglobulin M cerebrospinal fluid
Male
Middle Aged
Multiple Sclerosis blood
Multiple Sclerosis cerebrospinal fluid
Multiple Sclerosis diagnostic imaging
Nervous System Diseases blood
Nervous System Diseases cerebrospinal fluid
RNA, Viral cerebrospinal fluid
Retrospective Studies
Virus Latency genetics
Herpesvirus 6, Human isolation & purification
MicroRNAs blood
MicroRNAs cerebrospinal fluid
Multiple Sclerosis virology
RNA, Viral blood
Subjects
Details
- Language :
- English
- ISSN :
- 1664-3224
- Volume :
- 11
- Database :
- MEDLINE
- Journal :
- Frontiers in immunology
- Publication Type :
- Academic Journal
- Accession number :
- 33072077
- Full Text :
- https://doi.org/10.3389/fimmu.2020.02142