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The Long Noncoding RNA ZEB2-AS1 Contributes to Proliferation and Epithelial-to-Mesenchymal Transition of Osteosarcoma.
- Source :
-
Cancer biotherapy & radiopharmaceuticals [Cancer Biother Radiopharm] 2023 Nov; Vol. 38 (9), pp. 596-603. Date of Electronic Publication: 2020 Oct 21. - Publication Year :
- 2023
-
Abstract
- Background: Long non-coding RNA Zinc finger E-box binding homeobox 2 (ZEB2) antisense RNA 1 (ZEB2-AS1) has been shown to promote tumor progression. However, the clinical significance and fundamental function role of ZEB2-AS1 in osteosarcoma (OS) has been poorly understood. Methods: The expression of ZEB2-AS1 was determined in tumor tissues and matched normal tissues from 67 OS patients using quantitative reverse transcriptase PCR analysis. Clinical value of ZEB2-AS1 was evaluated by χ <superscript>2</superscript> test and Kaplan-Meier method. Cell proliferation was analyzed using CCK-8 assay, colony formation. Cell apoptosis status was determined by caspase-3 activity assay. Cell migration, invasion and epithelial-mesenchymal transition (EMT) were investigated by scratch wound healing, transwell invasion assays and Western blotting. Results: Clinical association analysis revealed that high ZEB2-AS1 expression correlated with tumor size, distant metastasis and poor prognosis of OS patients. Moreover, ZEB2-AS1 expression was identified as an independent prognostic factor for OS patients. Loss-of-function assays demonstrated that ZEB2-AS1 knockdown suppressed the proliferation and induced apoptosis in OS cells. In addition, ZEB2-AS1 knockdown inhibited cell migration, invasion, EMT of OS cells in vitro . Conclusions: Taken together, our data demonstrate that ZEB2-AS1 serves a putative oncogenic role and associates with unfavorable prognosis in OS.
- Subjects :
- Humans
Epithelial-Mesenchymal Transition genetics
Cell Line, Tumor
Cell Proliferation genetics
Cell Movement genetics
Gene Expression Regulation, Neoplastic
Zinc Finger E-box Binding Homeobox 2 genetics
Zinc Finger E-box Binding Homeobox 2 metabolism
RNA, Long Noncoding metabolism
Osteosarcoma pathology
Bone Neoplasms pathology
MicroRNAs
Subjects
Details
- Language :
- English
- ISSN :
- 1557-8852
- Volume :
- 38
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Cancer biotherapy & radiopharmaceuticals
- Publication Type :
- Academic Journal
- Accession number :
- 33085924
- Full Text :
- https://doi.org/10.1089/cbr.2019.3433