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Antibody Aggregate Removal Using a Mixed-Mode Chromatography Resin.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2178, pp. 345-354. - Publication Year :
- 2021
-
Abstract
- In monoclonal antibody (mAb) production, aggregates represent a major class of product-related impurities that needs to be removed by the downstream process. Protein A chromatography is generally less effective at removing antibody aggregates under typical conditions, and in most cases aggregate removal relies on a subsequent polishing chromatography. Here we describe a procedure for effective removal of antibody aggregates using the mixed-mode chromatography resin Capto MMC ImpRes. Clearance of aggregates was confirmed by analytical size-exclusion chromatography (SEC) and native gel electrophoresis.
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2178
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 33128760
- Full Text :
- https://doi.org/10.1007/978-1-0716-0775-6_23