Back to Search
Start Over
Upregulated ox40l Can Be Inhibited by miR-146a-5p in Condylar Chondrocytes Induced by IL-1β and TNF-α: A Possible Regulatory Mechanism in Osteoarthritis.
- Source :
-
International archives of allergy and immunology [Int Arch Allergy Immunol] 2021; Vol. 182 (5), pp. 408-416. Date of Electronic Publication: 2020 Nov 04. - Publication Year :
- 2021
-
Abstract
- Introduction: Osteoarthritis (OA) is a common musculoskeletal disease characterized by pain, stiffness, limited activity, occasional effusion, and local inflammation. MiR-146 is one of the noncoding RNA closely related to OA, but the role of miR-146 in OA remains controversial. The tumour necrosis factor receptor OX40 is activated by its cognate ligand OX40L (TNFSF4) and functions as a T-cell costimulatory molecule. The T-cell functions, including cytokine production, expansion, and survival, are enhanced by the OX40 costimulatory signals.<br />Methods: We established an inflammatory model of condylar chondrocytes induced by IL-1β and TNF-α and detected the expression of miRNA by miRNA sequencing. Then, cell transfection was used to study the role of miR146a-5p in OA. The Kyoto Encyclopedia of Genes and Genomes (KEGG) and database analysis were used to screen out potential target genes of miR-146a-5p. A dual luciferase activity assay tested whether ox40l is the target gene of miR-146a-5p.<br />Results: MiR-146a-5p and OX40L was upregulated after induced by IL-1β and TNF-α, miR-146a-5p reduced the production of inflammatory factors but had no effect on chondrophenotypic factors, and ox40l was targeted by miR-146a-5p.<br />Conclusion: OX40L and miR-146a-5p of condylar chondrocytes in the inflammatory environment (induced by IL-1β and TNF-α) were significantly increased, miR-146a-5p is a protective factor in the inflammatory response, which can reduce the production of inflammatory factors, and miR-146a-5p may regulate T-cell-mediated immunity through targeting of ox40l in OA.<br /> (© 2020 S. Karger AG, Basel.)
- Subjects :
- Chondrocytes pathology
Computational Biology methods
Disease Susceptibility
Gene Expression Profiling
Humans
OX40 Ligand metabolism
Osteoarthritis pathology
Chondrocytes metabolism
Gene Expression Regulation
Interleukin-1beta metabolism
OX40 Ligand genetics
Osteoarthritis genetics
Osteoarthritis metabolism
Tumor Necrosis Factor-alpha metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1423-0097
- Volume :
- 182
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- International archives of allergy and immunology
- Publication Type :
- Academic Journal
- Accession number :
- 33147588
- Full Text :
- https://doi.org/10.1159/000512291