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A real-time polymerase chain reaction-based approach for qualitative estimation of viral RNA in organ tissues of coxsackievirus A-16-infected neonatal mice.
- Source :
-
Indian journal of medical microbiology [Indian J Med Microbiol] 2020 Jul-Dec; Vol. 38 (3 & 4), pp. 464-468. - Publication Year :
- 2020
-
Abstract
- Hand, foot and mouth disease (HFMD) is a paediatric disease associated with enteroviruses (EVs). Among EVs, coxsackievirus A-16 (CVA-16) strain is currently in circulation and causing outbreaks in India. Neonatal mice (Institute of Cancer Research) strains were infected with CVA-16 strain isolated from HFMD patients to conduct pathological and molecular studies. Infected organs were harvested as per time points. A real-time polymerase chain reaction was used for qualitative estimation of viral RNA in organ tissues of infected mice. Skeletal muscle, brain tissue and cardiac tissues were the major target sites of CVA-16 tropism. The first-ever study was conducted on CVA-16 strains using the current approach in India.<br />Competing Interests: None
- Subjects :
- Animals
Animals, Newborn
Child, Preschool
Coxsackievirus Infections diagnosis
Enterovirus isolation & purification
Enterovirus pathogenicity
Feces virology
Humans
India
Infant
Mice
Mice, Inbred ICR
Pharynx virology
Rectum virology
Coxsackievirus Infections virology
Enterovirus genetics
RNA, Viral analysis
Real-Time Polymerase Chain Reaction
Subjects
Details
- Language :
- English
- ISSN :
- 1998-3646
- Volume :
- 38
- Issue :
- 3 & 4
- Database :
- MEDLINE
- Journal :
- Indian journal of medical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 33154265
- Full Text :
- https://doi.org/10.4103/ijmm.IJMM_20_117