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Quantification of Ammonia Oxidizing Bacterial Abundances in Environmental Samples by Quantitative-PCR.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2232, pp. 135-146. - Publication Year :
- 2021
-
Abstract
- Quantitative-PCR (qPCR) enables the quantification of specific DNA targets, such as functional or phylogenetic marker genes associated with environmental samples. During each qPCR cycle, the number of copies of a gene (or region) of interest in DNA samples is determined in real time using a fluorescence-based label and compared to a standard serial dilution. Here, we describe a qPCR method to quantify the ammonia oxidizing bacteria involved in the first step of nitrification, using the amoA gene as a proxy of their abundance. The preparation of the standards from environmental samples and qPCR is presented in detail for specifically quantifying microbial abundance in environmental samples such as soil.
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2232
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 33161545
- Full Text :
- https://doi.org/10.1007/978-1-0716-1040-4_12