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Vimentin protects differentiating stem cells from stress.
- Source :
-
Scientific reports [Sci Rep] 2020 Nov 11; Vol. 10 (1), pp. 19525. Date of Electronic Publication: 2020 Nov 11. - Publication Year :
- 2020
-
Abstract
- Vimentin is one of the first cytoplasmic intermediate filaments to be expressed in mammalian cells during embryogenesis, but its role in cellular fitness has long been a mystery. Vimentin is acknowledged to play a role in cell stiffness, cell motility, and cytoplasmic organization, yet it is widely considered to be dispensable for cellular function and organismal development. Here, we show that Vimentin plays a role in cellular stress response in differentiating cells, by recruiting aggregates, stress granules, and RNA-binding proteins, directing their elimination and asymmetric partitioning. In the absence of Vimentin, pluripotent embryonic stem cells fail to differentiate properly, with a pronounced deficiency in neuronal differentiation. Our results uncover a novel function for Vimentin, with important implications for development, tissue homeostasis, and in particular, stress response.
- Subjects :
- Animals
Cell Differentiation physiology
Cells, Cultured
DNA Helicases metabolism
Fibroblasts cytology
Fibroblasts physiology
Mice
Mice, Knockout
Neurons cytology
Poly-ADP-Ribose Binding Proteins metabolism
Protein Aggregates physiology
RNA Helicases metabolism
RNA Recognition Motif Proteins metabolism
RNA-Binding Proteins metabolism
Stress, Physiological
Vimentin genetics
Mouse Embryonic Stem Cells cytology
Mouse Embryonic Stem Cells physiology
Vimentin metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 33177544
- Full Text :
- https://doi.org/10.1038/s41598-020-76076-4