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Lufenuron induces reproductive toxicity and genotoxic effects in pregnant albino rats and their fetuses.
- Source :
-
Scientific reports [Sci Rep] 2020 Nov 11; Vol. 10 (1), pp. 19544. Date of Electronic Publication: 2020 Nov 11. - Publication Year :
- 2020
-
Abstract
- Insecticides and other agrochemicals have become indispensable components of the agricultural system to ensure a notable increase in crop yield and food production. As a natural consequence, chemical residues result in significantly increased contamination of both terrestrial and aquatic ecosystems. The present study evaluated the teratogenic, genotoxic, and oxidative stress effects of residual-level lufenuron exposure on pregnant rats during the organogenesis gestational period of both mother and fetus. The tested dams were divided into three groups; control (untreated), low-dose group (orally administered with 0.4 mg/kg lufenuron) and high-dose group (orally administered with 0.8 mg/kg lufenuron). The dams of the two treatment groups showed teratogenic abnormalities represented by the asymmetrical distribution of fetuses in both uterine horns, accompanied by observed resorption sites and intensive bleeding in the uterine horns, whereas their fetuses suffered from growth retardation, morphologic malformations, and skeletal deformations. Histologic examination of the liver and kidney tissues obtained from mothers and fetuses after lufenuron exposure revealed multiple histopathologic changes. DNA fragmentation and cell cycle perturbation were also detected in the liver cells of lufenuron-treated pregnant dams and their fetuses through comet assay and flow cytometry, respectively. Moreover, lufenuron-induced oxidative stress in the liver of mothers and fetuses was confirmed by the increased malondialdehyde levels and decreased levels of enzymatic antioxidants (glutathione peroxidase and superoxide dismutase). Taken together, it can be concluded that lufenuron has a great potential in exerting teratogenic, genotoxic, and oxidative stresses on pregnant rats and their fetuses upon chronic exposure to residual levels during the organogenesis gestational period. The obtained results in the present study imply that women and their fetuses may have the same risk.
- Subjects :
- Animals
Benzamides administration & dosage
Bone and Bones abnormalities
Bone and Bones drug effects
Cell Cycle drug effects
Dose-Response Relationship, Drug
Female
Fetus pathology
Hepatocytes drug effects
Hepatocytes pathology
Kidney drug effects
Kidney pathology
Liver embryology
Male
Mutagenicity Tests methods
Oxidative Stress drug effects
Pregnancy
Rats, Sprague-Dawley
Reproduction drug effects
Teratogens toxicity
Toxicity Tests methods
Uterus drug effects
Benzamides toxicity
Fetus drug effects
Liver drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 33177580
- Full Text :
- https://doi.org/10.1038/s41598-020-76638-6