Back to Search
Start Over
A multifunctional nanoparticle as a prophylactic and therapeutic approach targeting respiratory syncytial virus.
- Source :
-
Nanomedicine : nanotechnology, biology, and medicine [Nanomedicine] 2021 Feb; Vol. 32, pp. 102325. Date of Electronic Publication: 2020 Nov 10. - Publication Year :
- 2021
-
Abstract
- Respiratory Syncytial Virus (RSV) has been a major health concern globally for decades, yet no effective prophylactic or treatment regimen is available. The key viral proteins responsible for RSV pathology include the fusion protein (F), the immunomodulatory non-structural-protein 1 (NS1) and the phosphoprotein (P) involved in viral replication. Herein, we developed a novel shell-core multifunctional nanosystem with dual payload: a plasmid construct encoding for shRNAs against NS1 and P, and an anti-fusion peptide (HR2D). Anti-ICAM1 antibody conjugated on the nanoparticle (NP) surface is used to target RSV infected cells. Our data show the potential of this nanosystem as a prophylactic and/or a therapeutic regimen against RSV infection. Furthermore, therapy of RSV infected mice with this nanosystem, in addition to reducing viral load, modulated expression of Th2 and allergy-associated cytokines such as IL4, IL-13 and IL-17 indicating a direct role of this nanosystem in the mechanisms involved in the immunoregulation of disease pathogenesis.<br /> (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Cytokines metabolism
Drug Liberation
Female
Inflammation Mediators metabolism
Intercellular Adhesion Molecule-1 metabolism
Mice, Inbred BALB C
Multifunctional Nanoparticles ultrastructure
Peptides pharmacology
Plasmids genetics
RNA, Small Interfering metabolism
Respiratory Syncytial Virus Infections virology
Respiratory Syncytial Virus, Human drug effects
Transfection
Viral Fusion Proteins metabolism
Mice
Multifunctional Nanoparticles therapeutic use
Respiratory Syncytial Virus Infections prevention & control
Respiratory Syncytial Virus, Human physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1549-9642
- Volume :
- 32
- Database :
- MEDLINE
- Journal :
- Nanomedicine : nanotechnology, biology, and medicine
- Publication Type :
- Academic Journal
- Accession number :
- 33186695
- Full Text :
- https://doi.org/10.1016/j.nano.2020.102325