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Bile Ductal Transcriptome Identifies Key Pathways and Hub Genes in Clonorchis sinensis-Infected Sprague-Dawley Rats.
- Source :
-
The Korean journal of parasitology [Korean J Parasitol] 2020 Oct; Vol. 58 (5), pp. 513-525. Date of Electronic Publication: 2020 Oct 22. - Publication Year :
- 2020
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Abstract
- Clonorchis sinensis is a food-borne trematode that infects more than 15 million people. The liver fluke causes clonorchiasis and chronical cholangitis, and promotes cholangiocarcinoma. The underlying molecular pathogenesis occurring in the bile duct by the infection is little known. In this study, transcriptome profile in the bile ducts infected with C. sinensis were analyzed using microarray methods. Differentially expressed genes (DEGs) were 1,563 and 1,457 at 2 and 4 weeks after infection. Majority of the DEGs were temporally dysregulated at 2 weeks, but 519 DEGs showed monotonically changing expression patterns that formed seven distinct expression profiles. Protein-protein interaction (PPI) analysis of the DEG products revealed 5 sub-networks and 10 key hub proteins while weighted co-expression network analysis (WGCNA)-derived gene-gene interaction exhibited 16 co-expression modules and 13 key hub genes. The DEGs were significantly enriched in 16 Kyoto Encyclopedia of Genes and Genomes pathways, which were related to original systems, cellular process, environmental information processing, and human diseases. This study uncovered a global picture of gene expression profiles in the bile ducts infected with C. sinensis, and provided a set of potent predictive biomarkers for early diagnosis of clonorchiasis.
- Subjects :
- Animals
Early Diagnosis
Epistasis, Genetic
Rats, Sprague-Dawley
Bile Ducts parasitology
Bile Ducts pathology
Clonorchiasis diagnosis
Clonorchiasis parasitology
Clonorchis sinensis genetics
Gene Expression genetics
Helminth Proteins genetics
Helminth Proteins metabolism
Signal Transduction genetics
Transcriptome
Subjects
Details
- Language :
- English
- ISSN :
- 1738-0006
- Volume :
- 58
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- The Korean journal of parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 33202503
- Full Text :
- https://doi.org/10.3347/kjp.2020.58.5.513