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Validation of Pf SNP-LAMP-Lateral Flow Dipstick for Detection of Single Nucleotide Polymorphism Associated with Pyrimethamine Resistance in Plasmodium falciparum .

Authors :
Yongkiettrakul S
Kolié FR
Kongkasuriyachai D
Sattabongkot J
Nguitragool W
Nawattanapaibool N
Suansomjit C
Warit S
Kangwanrangsan N
Buates S
Source :
Diagnostics (Basel, Switzerland) [Diagnostics (Basel)] 2020 Nov 13; Vol. 10 (11). Date of Electronic Publication: 2020 Nov 13.
Publication Year :
2020

Abstract

The loop-mediated isothermal amplification coupled with lateral flow dipstick ( Pf SNP-LAMP-LFD) was recently developed to detect single nucleotide polymorphism (A A T → A T T), corresponding to substitution of asparagine to isoleucine at amino acid position 51 in the P. falciparum dhfr-ts gene associated with antifolate resistance. In this present study, the Pf SNP-LAMP-LFD was validated on 128 clinical malaria samples of broad ranged parasite densities (10 to 87,634 parasites per microliter of blood). The results showed 100% accuracy for the detection of single nucleotide polymorphism for N51I mutation. Indeed, the high prevalence of N51I in the Pfdhfr-ts gene detected in the clinical samples is in line with reports of widespread antifolate resistant P. falciparum in Thailand. The relationship between enzyme choice and reaction time was observed to have an effect on Pf SNP-LAMP-LFD specificity; however, the method yielded consistent results once the conditions have been optimized. The results demonstrate that Pf SNP-LAMP-LFD is a simple method with sufficient sensitivity and specificity to be deployed in routine surveillance of antifolate resistance molecular marker and inform antimalarial management policy.

Details

Language :
English
ISSN :
2075-4418
Volume :
10
Issue :
11
Database :
MEDLINE
Journal :
Diagnostics (Basel, Switzerland)
Publication Type :
Academic Journal
Accession number :
33202937
Full Text :
https://doi.org/10.3390/diagnostics10110948