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siRNA-mediated silencing of CD44 delivered by Jet Pei enhanced Doxorubicin chemo sensitivity and altered miRNA expression in human breast cancer cell line (MDA-MB468).
- Source :
-
Molecular biology reports [Mol Biol Rep] 2020 Dec; Vol. 47 (12), pp. 9541-9551. Date of Electronic Publication: 2020 Nov 18. - Publication Year :
- 2020
-
Abstract
- CD44, as a superficial cellular glycoprotein, is an essential factor in cell-cell and cell-matrix interaction. The CD44 expression level has been substantially up-regulated in breast cancer, and this upregulation facilitates tumor proliferation and angiogenesis. This study aims to evaluate the combination therapy of Jet Pei/CD44-specific-siRNA/doxorubicin in breast cancer MDA-MB468 cell line. The MTT assay, wound healing test, colony formation assay, DAPI staining, and flow cytometry were performed to investigate the tumoral cell viability, migration, clonogenesis, and apoptosis progression. The quantitative real-time PCR (qRT-PCR) was performed to demonstrate the CD44 expression level. Finally, the effect of CD44 silencing on the expression of VEGF, CXCR4, MMP9, and MiR-142-3p was measured. The combination of CD44-specific-siRNA with doxorubicin decreased tumoral metastasis, proliferation, invasion, and migration, and increased apoptosis in MDA-MB468 cells. In conclusions, CD44 can serve as a therapeutic target in breast cancer. Moreover, the combination therapy of CD44-specific-siRNA with doxorubicin can be a promising treatment for patients with breast cancer.
- Subjects :
- Apoptosis genetics
Cell Line, Tumor
Cell Movement drug effects
Cell Proliferation drug effects
Female
Humans
Hyaluronan Receptors genetics
Hyaluronan Receptors metabolism
MCF-7 Cells
Matrix Metalloproteinase 9 genetics
Matrix Metalloproteinase 9 metabolism
MicroRNAs genetics
MicroRNAs metabolism
Polyethyleneimine chemistry
RNA, Messenger genetics
RNA, Messenger metabolism
RNA, Small Interfering genetics
RNA, Small Interfering metabolism
Receptors, CXCR4 genetics
Receptors, CXCR4 metabolism
Signal Transduction
Vascular Endothelial Growth Factor A genetics
Vascular Endothelial Growth Factor A metabolism
Antibiotics, Antineoplastic pharmacology
Apoptosis drug effects
Doxorubicin pharmacology
Gene Expression Regulation, Neoplastic
Hyaluronan Receptors antagonists & inhibitors
RNA, Messenger antagonists & inhibitors
Transfection methods
Subjects
Details
- Language :
- English
- ISSN :
- 1573-4978
- Volume :
- 47
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Molecular biology reports
- Publication Type :
- Academic Journal
- Accession number :
- 33206362
- Full Text :
- https://doi.org/10.1007/s11033-020-05952-z