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Advances in long-wavelength native phasing at X-ray free-electron lasers.

Authors :
Nass K
Cheng R
Vera L
Mozzanica A
Redford S
Ozerov D
Basu S
James D
Knopp G
Cirelli C
Martiel I
Casadei C
Weinert T
Nogly P
Skopintsev P
Usov I
Leonarski F
Geng T
Rappas M
Doré AS
Cooke R
Nasrollahi Shirazi S
Dworkowski F
Sharpe M
Olieric N
Bacellar C
Bohinc R
Steinmetz MO
Schertler G
Abela R
Patthey L
Schmitt B
Hennig M
Standfuss J
Wang M
Milne CJ
Source :
IUCrJ [IUCrJ] 2020 Sep 09; Vol. 7 (Pt 6), pp. 965-975. Date of Electronic Publication: 2020 Sep 09 (Print Publication: 2020).
Publication Year :
2020

Abstract

Long-wavelength pulses from the Swiss X-ray free-electron laser (XFEL) have been used for de novo protein structure determination by native single-wavelength anomalous diffraction (native-SAD) phasing of serial femtosecond crystallography (SFX) data. In this work, sensitive anomalous data-quality indicators and model proteins were used to quantify improvements in native-SAD at XFELs such as utilization of longer wavelengths, careful experimental geometry optimization, and better post-refinement and partiality correction. Compared with studies using shorter wavelengths at other XFELs and older software versions, up to one order of magnitude reduction in the required number of indexed images for native-SAD was achieved, hence lowering sample consumption and beam-time requirements significantly. Improved data quality and higher anomalous signal facilitate so-far underutilized de novo structure determination of challenging proteins at XFELs. Improvements presented in this work can be used in other types of SFX experiments that require accurate measurements of weak signals, for example time-resolved studies.<br /> (© Karol Nass et al. 2020.)

Details

Language :
English
ISSN :
2052-2525
Volume :
7
Issue :
Pt 6
Database :
MEDLINE
Journal :
IUCrJ
Publication Type :
Academic Journal
Accession number :
33209311
Full Text :
https://doi.org/10.1107/S2052252520011379