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Development of a Rapid Focus Reduction Neutralization Test Assay for Measuring SARS-CoV-2 Neutralizing Antibodies.
- Source :
-
Current protocols in immunology [Curr Protoc Immunol] 2020 Dec; Vol. 131 (1), pp. e116. - Publication Year :
- 2020
-
Abstract
- SARS-CoV-2 is a recently emerged human coronavirus that has escalated to a pandemic. There are currently no approved vaccines for SARS-CoV-2, which causes severe respiratory illness or death. Defining the antibody response to SARS-CoV-2 will be essential for understanding disease progression, long-term immunity, and vaccine efficacy. Here we describe two methods for evaluating the neutralization capacity of SARS-CoV-2 antibodies. The basic protocol is a focus reduction neutralization test (FRNT), which involves immunostaining infected cells with a chromogen deposit readout. The alternate protocol is a modification of the FRNT that uses an infectious clone-derived SARS-CoV-2 virus expressing a fluorescent reporter. These protocols are adapted for use in a high-throughput setting, and are compatible with large-scale vaccine studies or clinical testing. © 2020 Wiley Periodicals LLC Basic Protocol: Focus reduction neutralization test Alternate Protocol: mNeonGreen-based focus reduction neutralization test (FRNT-mNG).<br /> (© 2020 Wiley Periodicals LLC.)
Details
- Language :
- English
- ISSN :
- 1934-368X
- Volume :
- 131
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Current protocols in immunology
- Publication Type :
- Academic Journal
- Accession number :
- 33215858
- Full Text :
- https://doi.org/10.1002/cpim.116