Back to Search
Start Over
Chloroform extract of Citrus unshiu Markovich peel induces apoptosis and inhibits stemness in HeLa human cervical cancer cells.
- Source :
-
Molecular medicine reports [Mol Med Rep] 2021 Jan; Vol. 23 (1). Date of Electronic Publication: 2020 Nov 25. - Publication Year :
- 2021
-
Abstract
- Cervical cancer is the second most common cancer among women worldwide. However, chemotherapies for this cancer often cause many side effects and chemoresistance. Citrus unshiu Markovich peel (CECU) has been used as a traditional medicine for the treatment of various diseases in East Asia. Recently, the anticancer activities and mechanisms of action of CECU extract have been reported in a number of different cancer cell types, but no study has evaluated the therapeutic effect of this natural product on cervical cancer cells. In the current study, the anticancer activity and the underlying molecular mechanism of the chloroform extract of CECU was investigated on HeLa human cervical cancer cells. The results showed that CECU effectively inhibited the proliferation and migration of HeLa cells. Treatment of cells with CECU led to cell cycle arrest at the G2/M phase and activation of extrinsic and intrinsic apoptotic pathways. Furthermore, the proliferation inhibitory effect of CECU was due to the inactivation of AKT and ERK signaling, upregulation of p53 and p21, and downregulation of cyclin B1 and cyclin D1, but not reactive oxygen species (ROS) generation. Furthermore, CECU inhibited the stem‑like features of HeLa cells by downregulating key cancer stemness biomarkers. Therefore, CECU may be an effective complementary and alternative medicine for the prevention and treatment of cervical cancer.
- Subjects :
- Chloroform chemistry
Female
HeLa Cells
Humans
Neoplasm Proteins metabolism
Plant Extracts chemistry
Uterine Cervical Neoplasms drug therapy
Uterine Cervical Neoplasms pathology
Apoptosis drug effects
Citrus chemistry
Fruit chemistry
G2 Phase Cell Cycle Checkpoints drug effects
M Phase Cell Cycle Checkpoints drug effects
MAP Kinase Signaling System drug effects
Plant Extracts pharmacology
Uterine Cervical Neoplasms metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1791-3004
- Volume :
- 23
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Molecular medicine reports
- Publication Type :
- Academic Journal
- Accession number :
- 33236129
- Full Text :
- https://doi.org/10.3892/mmr.2020.11727