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Bombyx mori nucleopolyhedrovirus downregulates transcription factor BmFoxO to elevate virus infection.
- Source :
-
Developmental and comparative immunology [Dev Comp Immunol] 2021 Mar; Vol. 116, pp. 103904. Date of Electronic Publication: 2020 Nov 24. - Publication Year :
- 2021
-
Abstract
- Forkhead-box O (FoxO) is the primary transcriptional effector of the insulin-like signaling pathway that enhances gluconeogenesis through transcriptional activation of PEPCK and G6Pase in mammals. We have previously demonstrated the involvement of phosphoenolpyruvate carboxykinase (BmPEPCK-2) in antiviral immunity against the multiplication of Bombyx mori nuclearpolyhedrosisvirus (BmNPV) in silkworm. Therefore, we speculated that BmFoxO might suppress BmNPV by regulating the expression of PEPCK in silkworm. In the present study, we found that the expression of BmFoxO decreased after BmNPV infection in Bombyx mori; this finding was consistent with BmPEPCK-2 expression. In addition, the expression of BmFoxO was altered, and it was found that reduced expression of BmFoxO (dsBmFoxO) downregulated the expression of BmPEPCK-2 and increased the viral fluorescence and content in silkworm embryonic cell line BmE cells, and vice versa. BmFoxO could upregulate the expression of BmPEPCK-2 by binding to the BmPEPCK-2 promoter. Moreover, overexpression of BmFoxO significantly increased the expression of autophagy genes ATG6/7/8 after infection with BmNPV, consistent with BmPEPCK-2. These results indicate that BmNPV downregulates transcription factor BmFoxO to elevate virus infection, and BmFoxO overexpression upregulates BmPEPCK-2 expression and enhances silkworm antiviral resistance.<br /> (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Autophagy genetics
Autophagy-Related Protein 7 genetics
Autophagy-Related Protein 7 metabolism
Autophagy-Related Protein 8 Family genetics
Autophagy-Related Protein 8 Family metabolism
Beclin-1 genetics
Beclin-1 metabolism
Bombyx metabolism
Bombyx virology
Cell Line
Forkhead Transcription Factors metabolism
Host-Pathogen Interactions
Insect Proteins metabolism
Nucleopolyhedroviruses physiology
Phosphoenolpyruvate Carboxykinase (ATP) genetics
Phosphoenolpyruvate Carboxykinase (ATP) metabolism
Promoter Regions, Genetic genetics
Protein Binding
Bombyx genetics
Down-Regulation
Forkhead Transcription Factors genetics
Gene Expression Regulation
Insect Proteins genetics
Nucleopolyhedroviruses growth & development
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0089
- Volume :
- 116
- Database :
- MEDLINE
- Journal :
- Developmental and comparative immunology
- Publication Type :
- Academic Journal
- Accession number :
- 33245980
- Full Text :
- https://doi.org/10.1016/j.dci.2020.103904