Back to Search
Start Over
Effect of Ocimum gratissimum Leaf-extract on Hematological Indices and Lipid Profile of Streptozotocin-induced Diabetic Wistar Rats.
- Source :
-
Pakistan journal of biological sciences : PJBS [Pak J Biol Sci] 2020 Jan; Vol. 23 (12), pp. 1523-1529. - Publication Year :
- 2020
-
Abstract
- Background and Objective: Ocimum gratissimum (OG) has been used in ethnopharmacology for the treatment of diabetes. The aim of the study was to evaluate the effect of Ocimum gratissimum leaf-extract on hematological indices and lipid profile of Streptozotocin-induced diabetic Wistar rats.<br />Materials and Methods: Twenty-four rats weighing 100-160 g were randomly assigned to four treatment groups, the normal and diabetic controls, received a placebo treatment, while groups three and four were administered glibenclamide and OG leaf-extract (400 mg kg-1 b.wt.), respectively. The extracts were administered twice daily for 28 days. The rats were sacrificed and whole blood was collected for hematological and serum lipid profile assays. Data were analyzed using one-way ANOVA.<br />Results: Diabetes induction resulted in decreases (p<0.05) in Red Blood Cell (RBC), Hemoglobin (Hb), White Blood Cell (WBC) and increases in Mean Corpuscular Hemoglobin and Blood platelets compared to the normal control. Treatment with O. gratissimum extract reversed RBC (7.74±0.39 μL), WBC (16.57±3.02) and Platelet (804.33±194.02) levels, but not Hb, towards normal levels (7.99±0.04, 11.27±0.69, 839.67±10.17 respectively). Diabetes induction also resulted in increases (p<0.05) in Triglyceride (TG) and Very-Low-Density Lipoprotein (VLDL), decreases (p<0.05) in High-Density Lipoprotein (HDL) and Low-Density Lipoprotein (LDL) compared to normal control with no significant change in Total Cholesterol (TC). After administration with Ocimum gratissimum TC, LDL and VLDL and HDL levels were significantly (p<0.05) reduced relative to the diabetic control. TG was however increased relative to the diabetic control.<br />Conclusion: Overall, data suggests the plant holds great potential in amelioration of diabetes-induced dyslipidemia and hematological disorders.
- Subjects :
- Animals
Biomarkers blood
Diabetes Mellitus, Experimental blood
Diabetes Mellitus, Experimental chemically induced
Dyslipidemias blood
Dyslipidemias chemically induced
Female
Hypoglycemic Agents isolation & purification
Hypolipidemic Agents isolation & purification
Male
Plant Extracts isolation & purification
Rats, Wistar
Streptozocin
Diabetes Mellitus, Experimental drug therapy
Dyslipidemias drug therapy
Hypoglycemic Agents pharmacology
Hypolipidemic Agents pharmacology
Lipids blood
Ocimum chemistry
Plant Extracts pharmacology
Plant Leaves chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1812-5735
- Volume :
- 23
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Pakistan journal of biological sciences : PJBS
- Publication Type :
- Academic Journal
- Accession number :
- 33274884
- Full Text :
- https://doi.org/10.3923/pjbs.2020.1523.1529