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A novel nanohybrid antimicrobial based on chitosan nanoparticles and antimicrobial peptide microcin J25 with low toxicity.
A novel nanohybrid antimicrobial based on chitosan nanoparticles and antimicrobial peptide microcin J25 with low toxicity.
- Source :
-
Carbohydrate polymers [Carbohydr Polym] 2021 Feb 01; Vol. 253, pp. 117309. Date of Electronic Publication: 2020 Oct 27. - Publication Year :
- 2021
-
Abstract
- Bacterial resistance to antibiotics is a critical public health concern. Alternatives of antibiotics are needed urgently. Herein, we designed and engineered a new nano-antimicrobial, chitosan nanoparticles (CNs)-antimicrobial peptide microcin J25 (MccJ25) conjugates (CNMs). The engineered CNMs proved to be highly active against Gram-negative and Gram-positive bacteria, and the activity of CNMs and CNs was stable in various thermal and pH environments. Escherichia coli K88 strain treated with CNMs did not acquire resistance in serial passage assays over a period of 18 days. Risk assessment with cell lines showed that CNMs did not cause toxicity. Additionally, CNMs did not reduce the lifespan of C. elegans. In summary, this study demonstrated that CNMs can serve as an excellent novel antimicrobial agent against multi-drug resistance pathogens.<br /> (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Caco-2 Cells
Caenorhabditis elegans drug effects
Cell Survival drug effects
Drug Resistance, Multiple, Bacterial drug effects
Escherichia coli drug effects
HEK293 Cells
Humans
Hydrogen-Ion Concentration
Longevity drug effects
Methicillin-Resistant Staphylococcus aureus drug effects
Microbial Sensitivity Tests
Particle Size
Risk Assessment
Temperature
Anti-Bacterial Agents chemistry
Bacteriocins chemistry
Chitosan chemistry
Nanoparticles chemistry
Pore Forming Cytotoxic Proteins chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1344
- Volume :
- 253
- Database :
- MEDLINE
- Journal :
- Carbohydrate polymers
- Publication Type :
- Academic Journal
- Accession number :
- 33278958
- Full Text :
- https://doi.org/10.1016/j.carbpol.2020.117309