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Specific determination of hepatitis B e antigen by antibodies targeting precore unique epitope facilitates clinical diagnosis and drug evaluation against hepatitis B virus infection.
- Source :
-
Emerging microbes & infections [Emerg Microbes Infect] 2021 Dec; Vol. 10 (1), pp. 37-50. - Publication Year :
- 2021
-
Abstract
- Hepatitis B e antigen (HBeAg) is a widely used marker both for chronic hepatitis B (CHB) clinical management and HBV-related basic research. However, due to its high amino acid sequence homology to hepatitis B core antigen (HBcAg), most of available anti-HBe antibodies are cross-reactive with HBcAg resulting in high interference against accurate measurement of the status and level of HBeAg. In the study, we generated several monoclonal antibodies (mAbs) targeting various epitopes on HBeAg and HBcAg. Among these mAbs, a novel mAb 16D9, which recognizes the SKLCLG (aa -10 to -5) motif on the N-terminal residues of HBeAg that is absent on HBcAg, exhibited excellent detection sensitivity and specificity in pairing with another 14A7 mAb targeting the HBeAg C-terminus (STLPETTVVRRRGR, aa141 to 154). Based on these two mAbs, we developed a novel chemiluminescent HBeAg immunoassay (NTR-HBeAg) which could detect HBeAg derived from various HBV genotypes. In contrast to widely used commercial assays, the NTR-HBeAg completely eliminated the cross-reactivity with secreted HBcAg from precore mutant (G1896A) virus in either cell culture or patient sera. The improved specificity of the NTR-HBeAg assay enables its applicability in cccDNA-targeting drug screening in cell culture systems and also provides an accurate tool for clinical HBeAg detection.
- Subjects :
- Amino Acid Motifs
Antibodies, Monoclonal analysis
Cell Culture Techniques
Cell Line
Epitopes immunology
Genotype
Hep G2 Cells
Hepatitis B Core Antigens chemistry
Hepatitis B Core Antigens immunology
Hepatitis B e Antigens immunology
Hepatitis B virus immunology
Hepatitis B, Chronic blood
Humans
Luminescent Measurements
Hepatitis B Antibodies analysis
Hepatitis B e Antigens chemistry
Hepatitis B virus genetics
Hepatitis B, Chronic immunology
Subjects
Details
- Language :
- English
- ISSN :
- 2222-1751
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Emerging microbes & infections
- Publication Type :
- Academic Journal
- Accession number :
- 33296295
- Full Text :
- https://doi.org/10.1080/22221751.2020.1862631