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In utero exposure to alloantigens primes alloimmunization to platelet transfusion in mice.
- Source :
-
Transfusion [Transfusion] 2021 Mar; Vol. 61 (3), pp. 687-691. Date of Electronic Publication: 2020 Dec 18. - Publication Year :
- 2021
-
Abstract
- Background: Platelet transfusions remain a mainstay of treatment for many patients with thrombocytopenia, but can lead to alloantibodies to Human Leukocyte Antigens (anti-HLA) resulting in inadequate responses to subsequent platelet transfusions (refractoriness), as well as complicate transplantation. Despite substantial decreases in alloimmunization with the implementation of leukoreduction, a significant percentage of patients still become alloimmunized following platelet transfusions. It remains unclear why some patients make anti-HLA antibodies, but others do not make anti-HLA antibodies even with chronic transfusion. Antecedent pregnancy correlates with risk of alloimmunization due to platelet transfusion in humans - however, isolation of pregnancy as a single variable is not possible in human populations.<br />Study Design and Methods: A tractable murine model of pregnancy and transfusion was engineered by breeding C57BL/6 (H-2 <superscript>b</superscript> ) dames with BALB/c (H-2 <superscript>d</superscript> ) sires. After pregnancy, female mice were transfused with leukoreduced platelets from F1 (H-2 <superscript>b/d</superscript> ) donors that expressed the same paternal major histocompatibility complex (MHC) H-2 <superscript>d</superscript> alloantigens as the sires. Control groups allowed isolation of pregnancy or transfusion alone as independent variables. Alloimmunization was determined by testing serum for antibodies to H-2 <superscript>d</superscript> MHC alloantigens.<br />Results: No alloantibodies were detected after pregnancy alone, or in response to transfusion of platelets alone; however, significant levels of alloantibodies were detected when pregnancy was followed by transfusion.<br />Conclusions: These findings isolate antecedent pregnancy as a causal contribution to increased frequencies of alloimmunization by subsequent platelet transfusion in mice and provide a platform for ongoing mechanistic investigation.<br /> (© 2020 AABB.)
Details
- Language :
- English
- ISSN :
- 1537-2995
- Volume :
- 61
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Transfusion
- Publication Type :
- Academic Journal
- Accession number :
- 33336414
- Full Text :
- https://doi.org/10.1111/trf.16224