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The Effect of Orally Supplemented Melatonin on Larval Performance and Skeletal Deformities in Farmed Gilthead Seabream ( Sparus aurata ).
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2020 Dec 16; Vol. 21 (24). Date of Electronic Publication: 2020 Dec 16. - Publication Year :
- 2020
-
Abstract
- The gilthead seabream larval rearing in continuous light is common in most Mediterranean hatcheries to stimulate larval length growth and increase food consumption. Several studies have shown that continuous light affects larval development and increases the prevalence of skeletal deformities. Melatonin is a crucial pineal neurohormone that displays daily secretion patterns, stimulates cell proliferation and embryonic development in Atlantic salmon and zebrafish, and improves osseointegration in mice and humans. However, no studies have examined the effects of orally supplemented melatonin on skeletal deformities in Sparus aurata larvae. We administered exogenous melatonin to gilthead seabream larvae via enriched rotifers and nauplii of Artemia. Exogenous melatonin induced bone deformities and stimulated parathyroid hormone-related protein-coding gene ( PTHrP ) mRNA expression. In addition to the melatonin-induced PTHrP high expression level, the recorded non coordinated function of skeletal muscle and bone during growth can be the fountainhead of bone deformities. Both myosin light chain 2 ( mlc2 ) and bone gamma-carboxyglutamate protein-coding gene ( bglap ) expression levels were significantly affected by melatonin administration in an inverse dose-response manner during the exogenous melatonin administration. This is the first study to report the effect of inducing melatonin bone deformities on Sparus aurata larvae reared under ordinary hatchery conditions.
- Subjects :
- Animals
Bone and Bones drug effects
Bone and Bones metabolism
Dietary Supplements
Fish Proteins genetics
Fish Proteins metabolism
Larva drug effects
Larva metabolism
Melatonin administration & dosage
Muscle, Skeletal growth & development
Muscle, Skeletal metabolism
Myosin Light Chains genetics
Myosin Light Chains metabolism
Osteocalcin genetics
Osteocalcin metabolism
Parathyroid Hormone-Related Protein genetics
Parathyroid Hormone-Related Protein metabolism
Sea Bream growth & development
Bone Development drug effects
Bone and Bones abnormalities
Melatonin toxicity
Sea Bream abnormalities
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 21
- Issue :
- 24
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 33339403
- Full Text :
- https://doi.org/10.3390/ijms21249597