Application of real-time PCR to significantly reduce the time to obtain recombinant MVA virus.

Authors :: Orlova OV
Glazkova DV
Tsyganova GM
Antoshkina IV
Mintaev RR
Tikhonov AS
Bogoslovskaya EV
Shipulin GA
Source :: Journal of virological methods [J Virol Methods] 2021 Mar; Vol. 289, pp. 114056. Date of Electronic Publication: 2020 Dec 24.
Publication Year :: 2021
Abstract: Obtaining a pure recombinant Modified Vaccinia Ankara (MVA) virus is a multistage, time-consuming procedure. We describe a novel single-tube real-time PCR which enables determination of the amount of wild type and recombinant viruses and their ratio in plaques. Use of the real-time PCR significantly reduce the time and efforts needed to obtain purified recombinant MVA. The new approach has been applied to generate recombinant MVAs encoding different SARS-COV-2 antigens.<br /> (Copyright © 2020 Elsevier B.V. All rights reserved.)

Subjects :: Animals
Cell Line
Humans
Real-Time Polymerase Chain Reaction
Antigens, Viral
Genetic Vectors
SARS-CoV-2 genetics
Vaccinia virus isolation & purification

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