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Proteomic profile of vitreous in patients with tubercular uveitis.

Authors :: Bansal R
Khan MM
Dasari S
Verma I
Goodlett DR
Manes NP
Nita-Lazar A
Sharma SP
Kumar A
Singh N
Chakraborti A
Gupta V
Dogra MR
Ram J
Gupta A
Source :: Tuberculosis (Edinburgh, Scotland) [Tuberculosis (Edinb)] 2021 Jan; Vol. 126, pp. 102036. Date of Electronic Publication: 2020 Dec 03.
Publication Year :: 2021
Abstract: Objective: To elucidate disease-specific host protein profile in vitreous fluid of patients with intraocular inflammation due to tubercular uveitis (TBU).<br />Methods: Vitreous samples from 13 patients with TBU (group A), 7 with non-TBU (group B) and 9 with no uveitis (group C) were analysed by shotgun proteomics using Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS). Differentially expressed proteins (DEPs) were subjected to pathway analysis using WEB-based Gene SeT Analysis Toolkit software.<br />Results: Compared to control groups (B + C combined), group A (TBU) displayed 32 (11 upregulated, 21 downregulated) DEPs, which revealed an upregulation of coagulation cascades, complement and classic pathways, and downregulation of metabolism of carbohydrates, gluconeogenesis, glucose metabolism and glycolysis/gluconeogenesis pathways. When compared to group B (non-TBU) alone, TBU displayed 58 DEPs (21 upregulated, 37 downregulated), with an upregulation of apoptosis, KRAS signaling, diabetes pathways, classic pathways, and downregulation of MTORC1 signaling, glycolysis/gluconeogenesis, and glucose metabolism.<br />Conclusion: This differential protein profile provides novel insights into the molecular mechanisms of TBU and a baseline to explore vitreous biomarkers to differentiate TBU from non-TBU, warranting future studies to identify and validate them as a diagnostic tool in TBU. The enriched pathways generate interesting hypotheses and drive further research.<br /> (Copyright © 2020 Elsevier Ltd. All rights reserved.)