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Widespread Prevalence of Plasmid-Mediated Colistin Resistance Gene mcr-1 in Escherichia coli from Père David's Deer in China.
- Source :
-
MSphere [mSphere] 2020 Dec 23; Vol. 5 (6). Date of Electronic Publication: 2020 Dec 23. - Publication Year :
- 2020
-
Abstract
- Père David's deer ( Elaphurus davidianus or milu) is an endangered species, and the prevalence of antimicrobial resistance (AMR) such as mcr-1 -positive strains among them has been unknown. In this study, we aimed to investigate the genomic characterizations of mcr-1 -positive strains and provide insight into the dissemination of AMR in nature reserve settings. Sixty-seven mcr-1 -positive Escherichia coli isolates from 97 fecal samples were identified by PCR and found resistant to colistin. The prevalence of β-lactam resistance was very high, and there were 64 mcr-1 -positive isolates containing β-lactamase genes. Transconjugants of 66 mcr-1 -positive isolates were acquired through conjugation experiments. PCR-based replicon typing (PBRT) showed that 44 strains harbored IncI2 mcr-1 -bearing plasmids, eight strains harbored IncX4 mcr-1 -carrying plasmids, and 14 strains harbored IncHI2 mcr-1 -positive plasmids. Notably, mcr-1 was located in the chromosome of LD27-1. Clonal dissemination and horizontal dissemination of mcr-1 by plasmids coexist. We first report the prevalence of plasmid-mediated mcr-1 in E. coli from Père David's deer in China. mcr-1 -bearing IncI2 plasmid was the most frequent plasmid type, and the first IncI2 plasmid harboring both bla <subscript>CTX-M-132</subscript> and mcr-1 is characterized here. Our results support the implication of Père David's deer as a potential reservoir for MCR-1-producing E. coli IMPORTANCE The mcr-1 gene is widely reported around the world and has been identified on various plasmids with different replicon types. Resistance to the last-line antibiotic colistin mediated by mcr-1 still represents a threat to global public health. Père David's deer is a highly endangered species originating in China, and many deer are currently being raised in captivity for gradual reintroduction to the wild. If this species carries AMR bacteria, it will pose a potential threat to the environment. Therefore, research on the dissemination of mcr-1 -positive E. coli from Père David's deer is of great significance. This is the first study to investigate the microbiological and genomic surveillance of MCR-1-producing bacteria colonized among Père David's deer in China, and we uncovered a high prevalence of MCR-1-producing E. coli The importance of constant surveillance for AMR bacteria in nature reserve settings is emphasized.<br /> (Copyright © 2020 Lu et al.)
- Subjects :
- Animals
Anti-Bacterial Agents pharmacology
China epidemiology
Colistin pharmacology
Drug Resistance, Bacterial
Escherichia coli isolation & purification
Feces microbiology
Genotype
Plasmids analysis
Deer
Escherichia coli enzymology
Escherichia coli genetics
Escherichia coli Proteins genetics
beta-Lactamases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2379-5042
- Volume :
- 5
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- MSphere
- Publication Type :
- Academic Journal
- Accession number :
- 33361130
- Full Text :
- https://doi.org/10.1128/mSphere.01221-20