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A mouse allergic asthma model induced by shrimp tropomyosin.
- Source :
-
International immunopharmacology [Int Immunopharmacol] 2021 Feb; Vol. 91, pp. 107289. Date of Electronic Publication: 2020 Dec 25. - Publication Year :
- 2021
-
Abstract
- Allergic asthma remains an important worldwide health issue. Animal models are valuable for understanding the pathophysiological mechanisms of asthma and the development of effective therapeutics. This study aims to develop an alternative murine model induced by shrimp tropomyosin (ST) instead of ovalbumin (OVA). To investigate responses to short-term exposure to antigens, mice were sensitized with intraperitoneal injections of ST or ST plus aluminum adjuvant on days 0, 7, 14 followed by an intranasal challenge with ST for seven consecutive days. We reveal that sensitization with ST alone or ST plus aluminum induces significant levels of serum total IgE and ST-specific IgE in mice. Challenge results show that ST causes severe eosinophilic airway inflammation. Histology analysis of the lung tissues demonstrates airway inflammation and mucus hypersecretion within the bronchi in mice exposed to ST. Analysis of the cell composition in bronchoalveolar lavage fluid (BALF) shows a significant increase in eosinophil count in ST alone and ST plus aluminum groups. We also detect increased CD4 <superscript>+</superscript> T lymphocytes in lung tissues and production of helper T cell type 2-associated cytokines (IL-4 and IL-5) in BALF. In addition, airway hyperresponsiveness to methacholine in ST alone and ST plus aluminum groups is much higher than that in control groups. For the chronic model, mice were sensitized by ST or ST plus aluminum adjuvant for 3weeks and challenged with ST for 6weeks. We find severe structural changes in animals upon prolonged exposure to ST, including goblet cell hyperplasia, collagen deposition, and smooth muscle thickening. In conclusion, ST-induced asthma is a simple murine model for studying pathogenesis of asthma and evaluating new therapeutic drugs.<br /> (Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Adjuvants, Immunologic
Animals
Asthma immunology
Asthma metabolism
Asthma pathology
Bronchial Hyperreactivity immunology
Bronchial Hyperreactivity metabolism
Bronchial Hyperreactivity physiopathology
Bronchoconstriction
Disease Models, Animal
Disease Progression
Female
Immunoglobulin E blood
Interleukin-4 metabolism
Interleukin-5 metabolism
Lung metabolism
Lung pathology
Lung physiopathology
Mice, Inbred C57BL
T-Lymphocytes, Helper-Inducer immunology
T-Lymphocytes, Helper-Inducer metabolism
Time Factors
Mice
Allergens
Asthma chemically induced
Bronchial Hyperreactivity chemically induced
Lung immunology
Penaeidae immunology
Tropomyosin
Subjects
Details
- Language :
- English
- ISSN :
- 1878-1705
- Volume :
- 91
- Database :
- MEDLINE
- Journal :
- International immunopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 33370683
- Full Text :
- https://doi.org/10.1016/j.intimp.2020.107289