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Influence of Hydroxyl Groups on the Cell Viability of Polyhydroxyalkanoate (PHA) Scaffolds for Tissue Engineering.
- Source :
-
ACS biomaterials science & engineering [ACS Biomater Sci Eng] 2017 Dec 11; Vol. 3 (12), pp. 3064-3075. Date of Electronic Publication: 2016 Sep 01. - Publication Year :
- 2017
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Abstract
- Polyhydroxyalkanoates (PHAs) are biopolyesters that have been studied as tissue engineering materials because of their biodegradability, biocompatibility, and low cytotoxicity. In this study, poly(3-hydroxybutyrate- co -3-hydroxyvalerate- co -2,3-dihydroxybutyrate) [PHBVDB] containing hydroxyl groups was produced by recombinant Ralstonia eutropha . R. eutropha were constructed to express the propionate-coenzymeA transferase ( pct ) gene from Megasphaera elsdenii , and glycolate was used as the carbon source. Disruption of phaA encoding β-ketothiolase in the phaCAB operon increased 2,3-dihydroxybutyrate (2,3-DHBA) compositions to 3 mol %. The PHBVDB film showed a lower water contact angle compared with other PHA films, indicating increased hydrophilicity due to the hydroxyl groups. The mechanical properties of the PHBVDB scaffold met the requirements for a soft tissue matrix. The effect of hydroxyl groups on cytotoxicity was evaluated with human mesenchymal stem cells. Results of cell proliferation and live/dead assays showed that the PHBVDB scaffold did not exhibit significant cytotoxicity toward the cells. These results indicate that PBVDB containing hydroxyl groups could be applied as a hydrophilicity-controlled scaffold for soft tissue engineering.
Details
- Language :
- English
- ISSN :
- 2373-9878
- Volume :
- 3
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- ACS biomaterials science & engineering
- Publication Type :
- Academic Journal
- Accession number :
- 33445351
- Full Text :
- https://doi.org/10.1021/acsbiomaterials.6b00279