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Development of immunoassay methods based on monoclonal antibody and its application in the determination of cadmium ion.
- Source :
-
Journal of hazardous materials [J Hazard Mater] 2021 Jun 05; Vol. 411, pp. 124992. Date of Electronic Publication: 2020 Dec 31. - Publication Year :
- 2021
-
Abstract
- Owing to the threat of cadmium (Cd <superscript>2+</superscript> ) to public health, it is an urgent demand to develop effective, sensitive, and rapid methods for the detection of cadmium. In this study, indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and immunochromatographic test strips (ICTS) were established for the determination of Cd <superscript>2+</superscript> based on the obtained mAb with high specificity and high affinity (Kaff = 3.0 × 10 <superscript>9</superscript> L/moL). The linear range of ic-ELISA detection was 0.03-1.11 ng/mL and 50% inhibitive concentration (IC50) of cadmium ion was determined to be 0.15 ng/mL. The visual limit of detection (vLOD) of the AuNS-based strip was 0.375 ng/mL. The vLOD of AuNF-based strip using higher intensity reporter determined to be 0.03 ng/mL, which was enhanced 12 times compared to the traditional strip. In summary, the developed immunoassays based on mAb shows great potential for monitoring the cadmium ion in environmental samples.<br /> (Copyright © 2021 Elsevier B.V. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1873-3336
- Volume :
- 411
- Database :
- MEDLINE
- Journal :
- Journal of hazardous materials
- Publication Type :
- Academic Journal
- Accession number :
- 33454572
- Full Text :
- https://doi.org/10.1016/j.jhazmat.2020.124992