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The role of Zur-regulated lipoprotein A in bacterial morphology, antimicrobial susceptibility, and production of outer membrane vesicles in Acinetobacter baumannii.
- Source :
-
BMC microbiology [BMC Microbiol] 2021 Jan 18; Vol. 21 (1), pp. 27. Date of Electronic Publication: 2021 Jan 18. - Publication Year :
- 2021
-
Abstract
- Background: Zinc uptake-regulator (Zur)-regulated lipoprotein A (ZrlA) plays a role in bacterial fitness and overcoming antimicrobial exposure in Acinetobacter baumannii. This study further characterized the zrlA gene and its encoded protein and investigated the roles of the zrlA gene in bacterial morphology, antimicrobial susceptibility, and production of outer membrane vesicles (OMVs) in A. baumannii ATCC 17978.<br />Results: In silico and polymerase chain reaction analyses showed that the zrlA gene was conserved among A. baumannii strains with 97-100% sequence homology. Recombinant ZrlA protein exhibited a specific enzymatic activity of D-alanine-D-alanine carboxypeptidase. Wild-type A. baumannii exhibited more morphological heterogeneity than a ΔzrlA mutant strain during stationary phase. The ΔzrlA mutant strain was more susceptible to gentamicin than the wild-type strain. Sizes and protein profiles of OMVs were similar between the wild-type and ΔzrlA mutant strains, but the ΔzrlA mutant strain produced 9.7 times more OMV particles than the wild-type strain. OMVs from the ΔzrlA mutant were more cytotoxic in cultured epithelial cells than OMVs from the wild-type strain.<br />Conclusions: The present study demonstrated that A. baumannii ZrlA contributes to bacterial morphogenesis and antimicrobial resistance, but its deletion increases OMV production and OMV-mediated host cell cytotoxicity.
- Subjects :
- A549 Cells
Acinetobacter baumannii drug effects
Acinetobacter baumannii genetics
Acinetobacter baumannii metabolism
Anti-Bacterial Agents pharmacology
Bacterial Proteins genetics
Bacterial Proteins metabolism
Computer Simulation
Extracellular Vesicles metabolism
Gentamicins pharmacology
Humans
Lipoprotein(a) metabolism
Mutation
Zinc metabolism
Acinetobacter baumannii pathogenicity
Computational Biology methods
Drug Resistance, Bacterial
Lipoprotein(a) genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1471-2180
- Volume :
- 21
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- BMC microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 33461493
- Full Text :
- https://doi.org/10.1186/s12866-020-02083-0