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ATI1 (ATG8-interacting protein 1) and ATI2 define a plant starvation-induced reticulophagy pathway and serve as MSBP1/MAPR5 cargo receptors.
- Source :
-
Autophagy [Autophagy] 2021 Nov; Vol. 17 (11), pp. 3375-3388. Date of Electronic Publication: 2021 Jan 25. - Publication Year :
- 2021
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Abstract
- Reticulophagy, the selective autophagy of endoplasmic reticulum (ER) components, is known to operate in eukaryotes from yeast and unicellular algae to animals and plants. Thus far, only ER-stress induced reticulophagy was reported and analyzed in plants. In this study we characterize a reticulophagy pathway in Arabidopsis thaliana that is triggered by dark-induced starvation but not by ER stress. This pathway is defined by the previously reported ATG8-interacting proteins, ATI1 and ATI2. We further identified the ER-localized MSBP1 (Membrane Steroid Binding Protein 1) as an ATI1- and ATI2-interacting protein and an autophagy cargo, and show that ATI1 and ATI2 serve as its cargo receptors. Together, these findings expand our knowledge on plant responses during energy deprivation and highlight the role of this special type of reticulophagy in this process. Abbreviations : AGO1: ARGONAUTE 1; ATI: ATG8-Interacting Protein; BiFC: Bimolecular Fluorescence Complementation; BR: brassinosteroid; conA: concanamycin A; DMSO: dimethyl sulfoxid; DTT: dithiothreitol; ER: endoplasmic reticulum; GFP: green fluorescent protein; MAPR: Membrane-Associated Progesterone Binding Protein; MSBP: Membrane Steroid Binding Protein; SD: standard deviation; SE: standard error; TM: tunicamycin; TOR: target of rapamycin; Y2H: yeast two-hybrid.
- Subjects :
- Arabidopsis cytology
Arabidopsis genetics
Arabidopsis metabolism
Arabidopsis Proteins genetics
Autophagy-Related Protein 8 Family metabolism
Endoplasmic Reticulum metabolism
Endoplasmic Reticulum Stress physiology
Membrane Proteins metabolism
Plants, Genetically Modified
Progesterone-Binding Globulin metabolism
Proteolysis
Vesicular Transport Proteins genetics
Arabidopsis Proteins metabolism
Autophagy physiology
Carrier Proteins metabolism
Vesicular Transport Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1554-8635
- Volume :
- 17
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Autophagy
- Publication Type :
- Academic Journal
- Accession number :
- 33487099
- Full Text :
- https://doi.org/10.1080/15548627.2021.1872886