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Blocking Interleukin-33 Alleviates the Joint Inflammation and Inhibits the Development of Collagen-Induced Arthritis in Mice.
- Source :
-
Journal of immunology research [J Immunol Res] 2020 Nov 05; Vol. 2020, pp. 4297354. Date of Electronic Publication: 2020 Nov 05 (Print Publication: 2020). - Publication Year :
- 2020
-
Abstract
- Rheumatoid arthritis (RA) is considered a systemic chronic inflammatory joint disease characterized by chronic synovitis and cartilage and bone destruction. Interleukin-33 (IL-33) is a proinflammatory cytokine which is highly expressed in the synovium of RA patients and the joints of mice with collagen-induced arthritis (CIA) and exacerbates CIA in mice. However, the role of the IL-33-neutralizing antibody in the murine model of CIA remains unclear. In the present study, CIA mice were given intraperitoneally with polyclonal rabbit anti-murine IL-33 antibody (anti-IL-33) or normal rabbit IgG control after the first signs of arthritis. Administration of anti-IL-33 after the onset of disease significantly reduced the severity of CIA and joint damage compared with controls treated with normal rabbit IgG. Anti-IL-33 treatment also significantly decreased the serum levels of interferon- γ (IFN- γ ),IL-6, IL-12, IL-33, and tumor necrosis factor- α (TNF- α ). Moreover, anti-IL-33 treatment significantly downregulated the production of IFN- γ , IL-6, IL-12, IL-33, and TNF- α in ex vivo-stimulated spleen cells. Together, our results indicate that the IL-33-neutralizing antibody may provide a therapeutic strategy for RA by inhibiting the release of proinflammatory cytokines.<br />Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.<br /> (Copyright © 2020 Yan Li et al.)
- Subjects :
- Animals
Arthritis, Experimental diagnosis
Arthritis, Experimental immunology
Arthritis, Experimental pathology
Arthritis, Rheumatoid diagnosis
Arthritis, Rheumatoid immunology
Arthritis, Rheumatoid pathology
Collagen administration & dosage
Collagen immunology
Cytokines immunology
Cytokines metabolism
Down-Regulation drug effects
Down-Regulation immunology
Humans
Injections, Intraperitoneal
Interleukin-33 metabolism
Joints immunology
Joints pathology
Male
Mice
Severity of Illness Index
Antibodies, Neutralizing administration & dosage
Arthritis, Experimental drug therapy
Arthritis, Rheumatoid drug therapy
Interleukin-33 antagonists & inhibitors
Subjects
Details
- Language :
- English
- ISSN :
- 2314-7156
- Volume :
- 2020
- Database :
- MEDLINE
- Journal :
- Journal of immunology research
- Publication Type :
- Academic Journal
- Accession number :
- 33490289
- Full Text :
- https://doi.org/10.1155/2020/4297354