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Genomic analysis of VIM-2-producing Enterobacter hormaechei subsp. steigerwaltii.
- Source :
-
International journal of antimicrobial agents [Int J Antimicrob Agents] 2021 Mar; Vol. 57 (3), pp. 106285. Date of Electronic Publication: 2021 Jan 22. - Publication Year :
- 2021
-
Abstract
- Carbapenemase-producing Enterobacterales (CPE) is a major public-health concern. Here we describe the occurrence of bla <subscript>VIM-2</subscript> in three isolates of Enterobacter hormaechei subsp. steigerwaltii. The bla <subscript>VIM-2</subscript> gene was part of a class II transposon Tn1332 and was embedded in a remnant of a class 1 integron. Tn1332 was carried by a large, conjugative, non-typeable plasmid. The three isolates belonged to sequence type 90 (ST90). Two isolates (90H2 and 90H3) were highly related [<10 single nucleotide polymorphisms (SNPs)], whereas isolate 104D2 exhibited more than 50 SNPs and Tn1332 was inserted in a different place in the plasmid. Another IncHI-type plasmid carrying the extended-spectrum β-lactamase (ESBL) gene bla <subscript>CTX-M-15</subscript> was identified in 90H2 and 90H3. Among the three isolates, isolate 104D2 was negative for detection of carbapenemase activity using the biochemical Carba NP test, despite the presence of Tn1332 on the same plasmid. Mutants of 104D2 with higher minimum inhibitory concentrations (MICs) for carbapenems were obtained and one mutant (m104D2) was analysed. In contrast to 104D2, mutant m104D2 gave a positive Carba NP test. The mutant possessed two copies of Tn1332 per cell and a nonsense mutation in WecA, an enzyme involved in enterobacterial common antigen and peptidoglycan intermediate biosynthesis. This study describes the first occurrence of Tn1332 in Enterobacterales and the phenotypic diversity of VIM-2-producing E. hormaechei.<br /> (Copyright © 2021 Elsevier Ltd and International Society of Antimicrobial Chemotherapy. All rights reserved.)
- Subjects :
- Bacterial Proteins genetics
Bacterial Proteins metabolism
Carbapenems pharmacology
DNA, Bacterial genetics
Drug Resistance, Bacterial
Enterobacteriaceae Infections epidemiology
France epidemiology
Humans
Integrons
Microbial Sensitivity Tests
Mutation
Phenotype
Phylogeny
Plasmids genetics
Polymorphism, Single Nucleotide
Anti-Bacterial Agents pharmacology
DNA Transposable Elements
Enterobacter drug effects
Enterobacter genetics
Enterobacteriaceae Infections microbiology
beta-Lactamases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7913
- Volume :
- 57
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- International journal of antimicrobial agents
- Publication Type :
- Academic Journal
- Accession number :
- 33493673
- Full Text :
- https://doi.org/10.1016/j.ijantimicag.2021.106285