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Antimicrobial and antibiofilm photodynamic therapy against vancomycin resistant Staphylococcus aureus (VRSA) induced infection in vitro and in vivo.
- Source :
-
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V [Eur J Pharm Biopharm] 2021 Mar; Vol. 160, pp. 65-76. Date of Electronic Publication: 2021 Jan 25. - Publication Year :
- 2021
-
Abstract
- Biofilm mediated infection caused by multi-drug resistant bacteria are difficult to treat since it protects the microorganisms by host defense system, making them resistant to antibiotics and other antimicrobial agents. Combating such type of nosocomial infection, especially in immunocompromised patients, is an urgent need and foremost challenge faced by clinicians. Therefore, antimicrobial photodynamic therapy (aPDT) has been intensely pursued as an alternative therapy for bacterial infections. aPDT leads to the generation of reactive oxygen species (ROS) that destroy bacterial cells in the presence of a photosensitizer, visible light and oxygen. Here, we elucidated a possibility of its clinical application by reducing the treatment time and exposing curcumin to 20 J/cm <superscript>2</superscript> of blue laser light, which corresponds to only 52 s to counteract vancomycin resistant Staphylococcus aureus (VRSA) both in vitro and in vivo. To understand the mechanism of action, the generation of total reactive oxygen species (ROS) was quantified by 2'-7'-dichlorofluorescein diacetate (DCFH-DA) and the type of phototoxicity was confirmed by fluorescence spectroscopic analysis. The data showed more production of singlet oxygen, indicating type-II phototoxicity. Different anti-biofilm assays (crystal violet and congo red assays) and microscopic studies were performed at sub-MIC concentration of curcumin followed by treatment with laser light against preformed biofilm of VRSA. The result showed significant reduction in the preformed biofilm formation. Finally, its therapeutic potential was validated in skin abrasion wistar rat model. The result showed significant inhibition of bacterial growth. Furthermore, immunomodulatory analysis with rat serum was performed. A significant reduction in expression of proinflammatory cytokines TNF-α and IL-6 were observed. Hence, we conclude that curcumin mediated aPDT with 20 J/cm <superscript>2</superscript> of blue laser treatment (for 52 s) could be used against multi-drug resistant bacterial infections and preformed biofilm formation as a potential therapeutic approach.<br /> (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Subjects :
- Administration, Cutaneous
Animals
Bacterial Load drug effects
Bacterial Load radiation effects
Biofilms drug effects
Biofilms growth & development
Biofilms radiation effects
Disease Models, Animal
Drug Resistance, Multiple, Bacterial
Humans
Lasers, Semiconductor
Male
Microbial Sensitivity Tests
Photochemotherapy instrumentation
Rats
Reactive Oxygen Species metabolism
Skin microbiology
Skin pathology
Staphylococcal Skin Infections microbiology
Staphylococcal Skin Infections pathology
Vancomycin-Resistant Staphylococcus aureus growth & development
Vancomycin-Resistant Staphylococcus aureus isolation & purification
Anti-Infective Agents administration & dosage
Curcumin administration & dosage
Photochemotherapy methods
Staphylococcal Skin Infections drug therapy
Vancomycin-Resistant Staphylococcus aureus drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3441
- Volume :
- 160
- Database :
- MEDLINE
- Journal :
- European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
- Publication Type :
- Academic Journal
- Accession number :
- 33508436
- Full Text :
- https://doi.org/10.1016/j.ejpb.2021.01.012