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Activation of Opioid Receptors Attenuates Ischemia/Reperfusion Injury in Skeletal Muscle Induced by Tourniquet Placement.
- Source :
-
Mediators of inflammation [Mediators Inflamm] 2021 Jan 15; Vol. 2021, pp. 6699499. Date of Electronic Publication: 2021 Jan 15 (Print Publication: 2021). - Publication Year :
- 2021
-
Abstract
- Method: Mice were randomly assigned to the sham, I/R, Oxy, and I/R with Oxy groups. Oxy was injected intraperitoneally 30 min before tourniquet placement. Morphological changes of the gastrocnemius muscle in these mice were assessed by hematoxylin-eosin (HE) staining and electron microscopy. Expression levels of TLR4, NF- κ B, SIRT1, and PGC-1 α in the skeletal muscles were detected by western blot. Blood TNF- α levels, gastrocnemius muscle contractile force, and ATP concentration were examined.<br />Results: Compared with the I/R group, Oxy pretreatment attenuated skeletal muscle damage, decreased serum TNF- α levels, and inhibited the expression levels of TLR4/NF- κ B in the gastrocnemius muscle. Furthermore, Oxy treatment significantly increased serum ATP levels and the contractility of the skeletal muscles. SIRT1 and PGC-1 α levels were significantly reduced in gastrocnemius muscle after I/R. Oxy pretreatment recovered these protein expression levels.<br />Conclusion: Tourniquet-induced acute limb I/R results in morphological and functional impairment in skeletal muscle. Pretreatment with Oxy attenuates skeletal muscle from acute I/R injury through inhibition of TLR4/NF- κ B-dependent inflammatory response and protects SIRT1/PGC-1 α -dependent mitochondrial function.<br />Competing Interests: The authors declare that there is no conflict of interest regarding the publication of this article.<br /> (Copyright © 2021 Yue-Xian Guo et al.)
- Subjects :
- Adenosine Triphosphate metabolism
Animals
Inflammation
Male
Mice
Mice, Inbred C57BL
Microscopy, Electron
Muscle, Skeletal injuries
NF-kappa B p50 Subunit metabolism
Perfusion
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha metabolism
Sirtuin 1 metabolism
Toll-Like Receptor 4 metabolism
Muscle Contraction
Muscle, Skeletal metabolism
Receptors, Opioid metabolism
Reperfusion Injury metabolism
Tourniquets
Subjects
Details
- Language :
- English
- ISSN :
- 1466-1861
- Volume :
- 2021
- Database :
- MEDLINE
- Journal :
- Mediators of inflammation
- Publication Type :
- Academic Journal
- Accession number :
- 33510583
- Full Text :
- https://doi.org/10.1155/2021/6699499