Back to Search
Start Over
Enhanced transforming growth factor-beta signaling and fibrosis in the pectoralis major muscle of broiler chickens affected by wooden breast myopathy.
- Source :
-
Poultry science [Poult Sci] 2021 Mar; Vol. 100 (3), pp. 100804. Date of Electronic Publication: 2020 Nov 04. - Publication Year :
- 2021
-
Abstract
- Fibrosis has also been recorded as a prominent pathological feature within wooden breast (WB) myopathy of broiler chickens. This study was conducted to evaluate the accumulation of fibril collagen, deposition of the extracellular matrix (ECM) components, and the underlying mechanism mediating the pathogenic fibrotic process in the pectoralis major (PM) muscle of WB-affected birds. Broiler chickens were categorized into the control and WB groups based on the evaluation of myopathic lesions. Results indicated that the total content and area of collagen in cross-sections of the PM muscle, as well as the augmented expression of collagen-I and fibronectin in the ECM, were greatly increased in birds with WB. Wooden breast myopathy upregulated expressions of transforming growth factor-beta (TGF-β) and the phosphorylation of Smad 2 and 3, thereby activating TGF-β-mediated Smad signaling pathway, which further enhanced the transcription of profibrotic mediators. In addition, regulators involved in collagen biosynthesis and cross-linking including prolyl 4-hydroxylase, lysyl oxidase, lysyl hydroxylase, and decorin were increased in the WB muscle. Finally, the expressions of both matrix metalloproteinases (MMP) and tissue inhibitor of metalloproteinases (TIMP) were increased in the WB muscle, which might be related with reduced ECM remodeling. Overall, WB myopathy induces severe fibrosis by enhancing ECM deposition and collagen cross-linking in the PM muscle of broiler chickens, possibly via the activation of TGF-β signaling and the dysregulation of the MMP and TIMP system.<br /> (Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Chickens genetics
Fibrosis genetics
Fibrosis physiopathology
Fibrosis veterinary
Muscular Diseases genetics
Muscular Diseases pathology
Muscular Diseases veterinary
Pectoralis Muscles physiopathology
Poultry Diseases genetics
Poultry Diseases pathology
Signal Transduction
Transforming Growth Factor beta genetics
Transforming Growth Factor beta metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1525-3171
- Volume :
- 100
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Poultry science
- Publication Type :
- Academic Journal
- Accession number :
- 33516474
- Full Text :
- https://doi.org/10.1016/j.psj.2020.10.058